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Blotting, Northern
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Definition of 'Blotting, Northern'

Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.

Common names: Blotting, Northern; Blottings, Northern; Northern Blottings; Northern Blotting; Blot, Northern; Blots, Northern; Northern Blots; Northern Blot

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Monday, November 23, 2009

Downregulation of p21-activated kinase-1 inhibits the growth of gastric cancer cells involving cyclin B1.

29 Nov 2009 p21-Activated kinase 1 (Pak1), a serine/threonine kinase, has been implicated in cytoskeletal remodelling, cell motility, apoptosis and transformation. However, the role of Pak1 in gastric cancer remains unclear. In this study, we detected Pak1 ... Read more...


Runx2 and bone morphogenic protein 2 regulate the expression of an alternative Lef1 transcript during osteoblast maturation.

30 Oct 2009 Lymphoid Enhancer Binding Factor (Lef) 1 is a transcriptional effector of the Wnt/Lrp5/beta-catenin signaling cascade, which regulates osteoblast differentiation, bone density, and skeletal strength. In this study, we describe the expression and ... Read more...


Ago-TNRC6 triggers microRNA-mediated decay by promoting two deadenylation steps.

16 Oct 2009 MicroRNAs (miRNAs) silence the expression of their mRNA targets mainly by promoting mRNA decay. The mechanism, kinetics and participating enzymes for miRNA-mediated decay in mammalian cells remain largely unclear. Combining the approaches of ... Read more...

 

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Technical information about 'Blotting, Northern'

Definition: Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.

Descriptor UI: D015152

Alternative terms: Blotting, Northern; Blottings, Northern; Northern Blottings; Northern Blotting; Blot, Northern; Blots, Northern; Northern Blots; Northern Blot;

Allowable Qualifiers: classification; economics; history; instrumentation; methods; standards; trends; utilization; veterinary; statistics & numerical data; ethics;

Tree Number: E05.196.181.400.454.655.100; E05.196.401.319.670.100; E05.196.680.100; E05.393.525.100; E05.601.140;

History Note: 91(89); was see under MOLECULAR PROBE TECHNIQUES 1989-90

Technical Notes: "northern" is not capitalized in titles or translations; DF: NORTHERN BLOT

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