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Cloning, Molecular
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Definition of 'Cloning, Molecular'

The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

Common names: Cloning, Molecular; Molecular Cloning

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Saturday, November 21, 2009

A novel domain in translational GTPase BipA mediates interaction with the 70S ribosome and influences GTP hydrolysis.

8 Nov 2009 BipA is a universally conserved prokaryotic GTPase that exhibits differential ribosome association in response to stress-related events. It is a member of the translation factor family of GTPases along with EF-G and LepA. BipA has five domains. The ... Read more...


Characterization of cancer-linked BRCA1-BRCT missense variants and their interaction with phosphoprotein targets.

30 Oct 2009 The breast cancer tumor suppressor protein BRCA1 is involved in DNA repair and cell cycle control. Mutations at the two C-terminal tandem (BRCT) repeats of BRCA1 detected in breast tumor patients were identified either to lower the stability of the ... Read more...


Anopheles gambiae alkaline phosphatase is a functional receptor of Bacillus thuringiensis jegathesan Cry11Ba toxin.

18 Oct 2009 Alkaline phosphatases (ALPs, EC 3.1.3.1) isolated from lepidopteran and dipteran species are identified as receptors for Cry1Ac and Cry11Aa toxins, respectively [Jurat-Fuentes, J. L., and Adang, M. J. (2004) Eur. J. Biochem. 7, 3127-3135; Fernandez, ... Read more...

 

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Technical information about 'Cloning, Molecular'

Definition: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

Descriptor UI: D003001

Alternative terms: Cloning, Molecular; Molecular Cloning;

Related Mesh Headings: DNA, Recombinant; Genetic Engineering; Recombinant Fusion Proteins; Cloning, Organism;

Allowable Qualifiers: drug effects; methods; radiation effects;

Tree Number: E05.393.220;

History Note: 80

Technical Notes: differentiate from CLONE CELLS and CLONING, ORGANISM; "clonal analysis": index under CLONE CELLS; DF: CLONING MOL or MOL CLONING

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