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Flow Cytometry
Research News and Information
Definition of 'Flow Cytometry'Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. |
Saturday, November 21, 2009
RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells.
29 Nov 2009
RhoB, a tumor suppressor, has emerged as an interesting cancer target, and extensive studies aimed at understanding its role in apoptosis have been performed. In our study, we investigated the involvement of RhoB-interacting molecules in apoptosis. ... Read more...
29 Nov 2009
Stage 4 neuroblastoma (NB) is a devastating childhood cancer whose poor outcome has remained essentially unchanged in the last 20 years. Receptor tyrosine kinases have important roles in the control of proliferation, differentiation and apoptosis of ... Read more...
29 Nov 2009
Transforming growth factor-beta (TGF-beta) is a cytokine with potent immunosuppressive effects and is overexpressed in many tumors. Therefore, development of molecules able to inhibit TGF-beta is of paramount importance to improve the efficacy of ... Read more...
Latest indexed articles for 'Flow Cytometry'
These are the very latest articles for this heading:
- RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells.
29 Nov 2009 - Activity of tyrosine kinase inhibitor Dasatinib in neuroblastoma cells in vitro and in orthotopic mouse model.
29 Nov 2009 - Peptide inhibitors of transforming growth factor-beta enhance the efficacy of antitumor immunotherapy.
29 Nov 2009 - Sphingosine kinase 1 inhibition sensitizes hormone-resistant prostate cancer to docetaxel.
29 Nov 2009 - Flow cytometric determination of Src phosphorylation in pediatric patients treated with dasatinib.
29 Nov 2009 - Radiation response of cancer stem-like cells from established human cell lines after sorting for surface markers.
13 Nov 2009 - Intrinsic resistance to the MEK1/2 inhibitor AZD6244 (ARRY-142886) is associated with weak ERK1/2 signalling and/or strong PI3K signalling in colorectal cancer cell lines.
13 Nov 2009 - Blockade of TRPC6 channels induced G2/M phase arrest and suppressed growth in human gastric cancer cells.
13 Nov 2009 - Distinct progression-associated expression of tumor and stromal MMPs in HaCaT skin SCCs correlates with onset of invasion.
13 Nov 2009 - Clinical and biological significance of CXCR5 expressed by prostate cancer specimens and cell lines.
13 Nov 2009 - FoxP3+ regulatory T cells are distinct from leukemia cells in HTLV-1-associated adult T-cell leukemia.
13 Nov 2009 - Identification of a chemokine receptor profile characteristic for mediastinal large B-cell lymphoma.
13 Nov 2009 - GPR37 surface expression enhancement via N-terminal truncation or protein-protein interactions.
Nov 2009 - Immune responses in patients with metastatic renal cell carcinoma treated with dendritic cells pulsed with tumor lysate.
30 Oct 2009 - A comparative study of transfection methods for RNA interference in bone marrow-derived murine dendritic cells.
30 Oct 2009 - Effects of lipopolysaccharide on the induction of mixed chimerism in cyclophosphamide-induced tolerance.
30 Oct 2009 - Evaluation of cell surface expression of phosphatidylserine in ovarian carcinoma effusions using the annexin-V/7-AAD assay: clinical relevance and comparison with other apoptosis parameters.
30 Oct 2009 - Significant CD5 expression on normal stage 3 hematogones and mature B Lymphocytes in bone marrow.
30 Oct 2009 - Flow cytometric immunophenotyping and minimal residual disease analysis in multiple myeloma.
30 Oct 2009 - Immunophenotypic variations in mantle cell lymphoma.
30 Oct 2009
See a longer list of these articles.
Technical information about 'Flow Cytometry'
Definition: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Descriptor UI: D005434
Alternative terms: Flow Cytometry; Cytometry, Flow; Cytometries, Flow; Flow Cytometries; Microfluorometry, Flow; Flow Microfluorometries; Flow Microfluorometry; Microfluorometries, Flow; Flow Microfluorimetry; Microfluorimetry, Flow; Cytofluorometry, Flow; Cytofluorometries, Flow; Flow Cytofluorometries; Flow Cytofluorometry; Fluorescence-Activated Cell Sorting; Cell Sorting, Fluorescence-Activated; Cell Sortings, Fluorescence-Activated; Fluorescence Activated Cell Sorting; Fluorescence-Activated Cell Sortings; Sorting, Fluorescence-Activated Cell; Sortings, Fluorescence-Activated Cell;
Allowable Qualifiers: classification; economics; history; instrumentation; methods; standards; trends; utilization; veterinary; statistics & numerical data; ethics;
Tree Number: E05.196.712.516.600.240.350; E05.200.500.386.350;
Online Note: use FLUOROIMMUNOASSAY to search FLOW MICROFLUORIMETRY 1989-90
History Note: 82; FLOW MICROFLUORIMETRY was see FLUOROIMMUNOASSAY 1989-90
Technical Notes: flow cell sorting & FLUORESCENCE-ACTIVATED CELL SORTING: coord with CELL SEPARATION