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Flow Cytometry
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Definition of 'Flow Cytometry'

Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.

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Saturday, November 21, 2009

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Technical information about 'Flow Cytometry'

Definition: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.

Descriptor UI: D005434

Alternative terms: Flow Cytometry; Cytometry, Flow; Cytometries, Flow; Flow Cytometries; Microfluorometry, Flow; Flow Microfluorometries; Flow Microfluorometry; Microfluorometries, Flow; Flow Microfluorimetry; Microfluorimetry, Flow; Cytofluorometry, Flow; Cytofluorometries, Flow; Flow Cytofluorometries; Flow Cytofluorometry; Fluorescence-Activated Cell Sorting; Cell Sorting, Fluorescence-Activated; Cell Sortings, Fluorescence-Activated; Fluorescence Activated Cell Sorting; Fluorescence-Activated Cell Sortings; Sorting, Fluorescence-Activated Cell; Sortings, Fluorescence-Activated Cell;

Allowable Qualifiers: classification; economics; history; instrumentation; methods; standards; trends; utilization; veterinary; statistics & numerical data; ethics;

Tree Number: E05.196.712.516.600.240.350; E05.200.500.386.350;

Online Note: use FLUOROIMMUNOASSAY to search FLOW MICROFLUORIMETRY 1989-90

History Note: 82; FLOW MICROFLUORIMETRY was see FLUOROIMMUNOASSAY 1989-90

Technical Notes: flow cell sorting & FLUORESCENCE-ACTIVATED CELL SORTING: coord with CELL SEPARATION

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