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In Situ Nick-End Labeling - Methods
Research News and Information
Definition of 'In Situ Nick-End Labeling'An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION. |
Sunday, November 22, 2009
30 Oct 2009 
To accurately quantify the number of single-strand breaks (SSBs) induced in plasmid DNA molecules after irradiation, a new type of assay methodology has been explored. The new method is based on the TUNEL (terminal deoxynucleotide transferase dUTP ... Read more...
4 Sep 2009 Integrins regulate adhesion-dependent growth, survival and invasion of tumor cells. In particular, expression of integrin alpha(v)beta(3) is associated with progression of a variety of human tumors. Here we reveal a previously undescribed ... Read more...
EFHC1 interacts with microtubules to regulate cell division and cortical development.
4 Sep 2009 Mutations in the EFHC1 gene are linked to juvenile myoclonic epilepsy (JME), one of the most frequent forms of idiopathic generalized epilepsies. JME is associated with subtle alterations of cortical and subcortical architecture, but the underlying ... Read more...
Latest indexed articles for 'In Situ Nick-End Labeling - Methods'
These are the very latest articles for this heading:
- Quantification of radiation-induced single-strand breaks in plasmid DNA using a TUNEL/ELISA-based assay. 30 Oct 2009
- An integrin alpha(v)beta(3)-c-Src oncogenic unit promotes anchorage-independence and tumor progression. 4 Sep 2009
- EFHC1 interacts with microtubules to regulate cell division and cortical development. 4 Sep 2009
- Hippocampal development and neural stem cell maintenance require Sox2-dependent regulation of Shh. 4 Sep 2009
- The neurokinin-1 receptor modulates the methamphetamine-induced striatal apoptosis and nitric oxide formation in mice. 11 Aug 2009
- Fibroblast growth factor-9 inhibits astrocyte differentiation of adult mouse neural progenitor cells. 30 Jul 2009
- Protective effects of topiramate against hyperoxic brain injury in the developing brain. 26 Jul 2009
- Docosahexaenoic acid attenuates microglial activation and delays early retinal degeneration. 15 Jul 2009
- Inhibition of calpain attenuates encephalitogenicity of MBP-specific T cells. 15 Jul 2009
- BDNF-mediated cerebellar granule cell development is impaired in mice null for CaMKK2 or CaMKIV. 13 Jul 2009
- Tissue kallikrein protects cortical neurons against in vitro ischemia-acidosis/reperfusion-induced injury through the ERK1/2 pathway. 30 Jun 2009
- Identification of neural programmed cell death through the detection of DNA fragmentation in situ and by PCR. 29 Jun 2009
- Neuroendocrine transcriptional programs adapt dynamically to the supply and demand for neuropeptides as revealed in NSF mutant zebrafish.
21 Jun 2009 - Deletion of astrocyte connexins 43 and 30 leads to a dysmyelinating phenotype and hippocampal CA1 vacuolation. 15 Jun 2009
- Regulation of radial glial survival by signals from the meninges. 15 Jun 2009
- Sensory axon-derived neuregulin-1 is required for axoglial signaling and normal sensory function but not for long-term axon maintenance. 15 Jun 2009
- Cadmium exposure induces mitochondria-dependent apoptosis in oligodendrocytes. 9 Jun 2009
- Relationship between transmembrane signal transduction pathway and DNA repair and the mechanism after global cerebral ischemia-reperfusion in rats. 30 May 2009
- Cell cycle inhibition attenuates microglial proliferation and production of IL-1beta, MIP-1alpha, and NO after focal cerebral ischemia in the rat. 30 May 2009
- Cerebellar development in a baboon model of preterm delivery: impact of specific ventilatory regimes. 30 May 2009
See a longer list of these articles.
Technical information about 'In Situ Nick-End Labeling'
Definition: An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.
Descriptor UI: D020287
Alternative terms: In Situ Nick-End Labeling; TUNEL; Nick-End Labeling, In Situ; In Situ Nick End Labelling; In Situ Nick End Labeling; In Situ Nick End-Labelling; In Situ Nick-End Labelling; Nick End-Labeling, In Situ; Nick End Labeling, In Situ; Nick End-Labelling, In Situ; Nick End Labelling, In Situ; Nick-End Labelling, In Situ; In Situ Nick End-Labeling;
Related Mesh Headings: Apoptosis; Comet Assay;
Allowable Qualifiers: classification; economics; history; instrumentation; methods; standards; trends; utilization; veterinary; statistics & numerical data; ethics;
Tree Number: E05.393.475;
History Note: 99