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Mice, Knockout
Research News and Information
Definition of 'Mice, Knockout'Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes. |
Saturday, November 21, 2009
Matrix association of latent TGF-beta binding protein-2 (LTBP-2) is dependent on fibrillin-1.
29 Nov 2009 
The components of the extracellular matrix (ECM) and their differential expression patterns play important roles in tissue formation. The deposition of latent TGF-beta binding proteins (LTBPs) to the ECM exhibit distinct distribution profiles. We ... Read more...
29 Nov 2009 Apc-deficient Min mice feature low expression of lipoprotein lipase (LPL), high concentration of serum triglyceride (TG), fatty change of the liver and large numbers of intestinal polyps. We have reported that induction of LPL expression reduces ... Read more...
29 Nov 2009 Transforming growth factor-beta (TGF-beta) is a cytokine with potent immunosuppressive effects and is overexpressed in many tumors. Therefore, development of molecules able to inhibit TGF-beta is of paramount importance to improve the efficacy of ... Read more...
Latest indexed articles for 'Mice, Knockout'
These are the very latest articles for this heading:
- Matrix association of latent TGF-beta binding protein-2 (LTBP-2) is dependent on fibrillin-1. 29 Nov 2009
- Overexpression of low-density lipoprotein receptor and lipid accumulation in intestinal polyps in Min mice. 29 Nov 2009
- Peptide inhibitors of transforming growth factor-beta enhance the efficacy of antitumor immunotherapy. 29 Nov 2009
- Expression and role of CXCL10 during the encephalitic stage of experimental and clinical African trypanosomiasis. 13 Nov 2009
- The reduction of cell death and proliferation by p27(Kip1) minimizes DNA damage in an experimental model of genotoxicity. 13 Nov 2009
- Inositol 1,4,5-trisphosphate 3-kinase a functions as a scaffold for synaptic Rac signaling. 2 Nov 2009
- Phosphorylation of ezrin/radixin/moesin proteins by LRRK2 promotes the rearrangement of actin cytoskeleton in neuronal morphogenesis. 2 Nov 2009
- Autocrine and paracrine roles for ATP and serotonin in mouse taste buds. 2 Nov 2009
- Neuroligin-2 deletion selectively decreases inhibitory synaptic transmission originating from fast-spiking but not from somatostatin-positive interneurons. 2 Nov 2009
- Reticulon-4A (Nogo-A) redistributes protein disulfide isomerase to protect mice from SOD1-dependent amyotrophic lateral sclerosis. 2 Nov 2009
- Decreased brain edema after collagenase-induced intracerebral hemorrhage in mice lacking the inducible nitric oxide synthase gene. Laboratory investigation. 30 Oct 2009
- Insulin2 gene (Ins2) transcription by NOD bone marrow-derived cells does not influence autoimmune diabetes development in NOD-Ins2 knockout mice. 30 Oct 2009
- Reward-seeking and discrimination deficits displayed by hypodopaminergic mice are prevented in mice lacking dopamine D4 receptors. 30 Oct 2009
- Comparative analysis of cell killing and autosomal mutation in mouse kidney epithelium exposed to 1 GeV/nucleon iron ions in vitro or in situ. 30 Oct 2009
- S-phase kinase-associated protein-2 (Skp2) promotes vascular smooth muscle cell proliferation and neointima formation in vivo. 30 Oct 2009
- CCR5 down-regulates osteoclast function in orthodontic tooth movement. 30 Oct 2009
- The PPARalpha agonist fenofibrate preserves hippocampal neurogenesis and inhibits microglial activation after whole-brain irradiation. 30 Oct 2009
- ELKS2alpha/CAST deletion selectively increases neurotransmitter release at inhibitory synapses. 27 Oct 2009
- Spatial-temporal patterns of retinal waves underlying activity-dependent refinement of retinofugal projections. 27 Oct 2009
- A homozygous CARD9 mutation in a family with susceptibility to fungal infections. 27 Oct 2009
See a longer list of these articles.
Technical information about 'Mice, Knockout'
Definition: Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Descriptor UI: D018345
Alternative terms: Mice, Knockout; Mouse, Knockout; Knockout Mouse; Knockout Mice; Mice, Knock-out; Knock-out Mice; Mice, Knock out;
Related Mesh Headings: Gene Knockout Techniques;
Allowable Qualifiers: abnormalities; anatomy & histology; blood; cerebrospinal fluid; classification; embryology; genetics; growth & development; immunology; injuries; metabolism; microbiology; parasitology; physiology; psychology; surgery; urine; virology;
Tree Number: B01.050.680.136.500.500; B01.150.900.649.865.635.505.500.550.455; B01.150.900.649.865.635.505.500.800.500;
History Note: 94
Technical Notes: NIM no qualif when exper animal; do not forget also to check MICE