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Patch-Clamp Techniques - Methods
Research News and Information
Definition of 'Patch-Clamp Techniques'An electrophysiologic technique for studying cells, cell membranes, and occasionally isolated organelles. All patch-clamp methods rely on a very high-resistance seal between a micropipette and a membrane; the seal is usually attained by gentle suction. The four most common variants include on-cell patch, inside-out patch, outside-out patch, and whole-cell clamp. Patch-clamp methods are commonly used to voltage clamp, that is control the voltage across the membrane and measure current flow, but current-clamp methods, in which the current is controlled and the voltage is measured, are also used. |
Saturday, November 21, 2009
27 Oct 2009 
Theta oscillations are believed to play an important role in the coordination of neuronal firing in the entorhinal (EC)-hippocampal system but the underlying mechanisms are not known. We simultaneously recorded from neurons in multiple regions of ... Read more...
ELKS2alpha/CAST deletion selectively increases neurotransmitter release at inhibitory synapses.
27 Oct 2009 The presynaptic active zone is composed of a protein network that contains ELKS2alpha (a.k.a. CAST) as a central component. Here we demonstrate that in mice, deletion of ELKS2alpha caused a large increase in inhibitory, but not excitatory, ... Read more...
19 Oct 2009 Pure NMDA receptor (NMDAr)-mediated EPSCs, thought to correspond to "silent" glutamatergic synapses that lack AMPA receptors (AMPArs), have been observed in superficial spinal dorsal horn of neonatal but not adult rats. Recent anatomical studies ... Read more...
Latest indexed articles for 'Patch-Clamp Techniques - Methods'
These are the very latest articles for this heading:
- Theta oscillations provide temporal windows for local circuit computation in the entorhinal-hippocampal loop. 27 Oct 2009
- ELKS2alpha/CAST deletion selectively increases neurotransmitter release at inhibitory synapses. 27 Oct 2009
- Evidence against AMPA receptor-lacking glutamatergic synapses in the superficial dorsal horn of the rat spinal cord. 19 Oct 2009
- Kv7/KCNQ channels control action potential phasing of pyramidal neurons during hippocampal gamma oscillations in vitro. 19 Oct 2009
- The time course of dopamine transmission in the ventral tegmental area. 19 Oct 2009
- In vivo role of a potassium channel-binding protein in regulating neuronal excitability and behavior. 19 Oct 2009
- Nitric oxide potentiation of locomotor activity in the spinal cord of the lamprey. 19 Oct 2009
- Protein kinase C mediates the synergistic interaction between agonists acting at alpha2-adrenergic and delta-opioid receptors in spinal cord. 19 Oct 2009
- Endocannabinoid-dependent homeostatic regulation of inhibitory synapses by miniature excitatory synaptic activities. 19 Oct 2009
- Functional recovery after lesion of a central pattern generator. 19 Oct 2009
- T-type calcium channel inhibition underlies the analgesic effects of the endogenous lipoamino acids. 19 Oct 2009
- Nonlinear integration of visual and haltere inputs in fly neck motor neurons. 19 Oct 2009
- Mechanisms underlying directional selectivity for frequency-modulated sweeps in the inferior colliculus revealed by in vivo whole-cell recordings. 12 Oct 2009
- Cytoskeletal changes underlie estrogen's acute effects on synaptic transmission and plasticity. 12 Oct 2009
- Inhibitor kappaB Kinase beta deficiency in primary nociceptive neurons increases TRP channel sensitivity. 12 Oct 2009
- Retrograde viral vector-mediated inhibition of pontospinal noradrenergic neurons causes hyperalgesia in rats. 12 Oct 2009
- Differential effects of topiramate on prefrontal glutamatergic transmission when combined with raclopride or clozapine. 29 Sep 2009
- Dynamics of fast and slow inhibition from cerebellar golgi cells allow flexible control of synaptic integration. 22 Sep 2009
- High-affinity kainate receptor subunits are necessary for ionotropic but not metabotropic signaling. 22 Sep 2009
- Differential involvement of beta3 integrin in pre- and postsynaptic forms of adaptation to chronic activity deprivation. 14 Sep 2009
See a longer list of these articles.
Technical information about 'Patch-Clamp Techniques'
Definition: An electrophysiologic technique for studying cells, cell membranes, and occasionally isolated organelles. All patch-clamp methods rely on a very high-resistance seal between a micropipette and a membrane; the seal is usually attained by gentle suction. The four most common variants include on-cell patch, inside-out patch, outside-out patch, and whole-cell clamp. Patch-clamp methods are commonly used to voltage clamp, that is control the voltage across the membrane and measure current flow, but current-clamp methods, in which the current is controlled and the voltage is measured, are also used.
Descriptor UI: D018408
Alternative terms: Patch-Clamp Techniques; Patch Clamp Techniques; Patch-Clamp Technique; Technique, Patch-Clamp; Techniques, Patch-Clamp; Patch-Clamp Technics; Patch Clamp Technics; Patch-Clamp Technic; Technic, Patch-Clamp; Technics, Patch-Clamp; Whole-Cell Recording; Recording, Whole-Cell; Recordings, Whole-Cell; Whole Cell Recording; Whole-Cell Recordings; Voltage-Clamp Techniques; Technique, Voltage-Clamp; Techniques, Voltage-Clamp; Voltage Clamp Techniques; Voltage-Clamp Technique; Voltage-Clamp Technics; Technic, Voltage-Clamp; Technics, Voltage-Clamp; Voltage Clamp Technics; Voltage-Clamp Technic;
Allowable Qualifiers: classification; economics; history; instrumentation; methods; standards; trends; utilization; veterinary; statistics & numerical data; ethics;
Tree Number: E05.200.500.800;
History Note: 95
Technical Notes: a cytol technique for removing a tiny patch of plasma membrane; NIM