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Patch-Clamp Techniques
Research News and Information
Definition of 'Patch-Clamp Techniques'An electrophysiologic technique for studying cells, cell membranes, and occasionally isolated organelles. All patch-clamp methods rely on a very high-resistance seal between a micropipette and a membrane; the seal is usually attained by gentle suction. The four most common variants include on-cell patch, inside-out patch, outside-out patch, and whole-cell clamp. Patch-clamp methods are commonly used to voltage clamp, that is control the voltage across the membrane and measure current flow, but current-clamp methods, in which the current is controlled and the voltage is measured, are also used. |
Saturday, November 21, 2009
30 Oct 2009 
Rundown is ubiquitously seen in response to repetitive activation of receptor or ion channels as a use-dependent down-regulation through various mechanisms. In contrast to AMPA receptors, gamma-aminobutyric acid type A receptor (GABA(A)R) are ... Read more...
27 Oct 2009 Theta oscillations are believed to play an important role in the coordination of neuronal firing in the entorhinal (EC)-hippocampal system but the underlying mechanisms are not known. We simultaneously recorded from neurons in multiple regions of ... Read more...
ELKS2alpha/CAST deletion selectively increases neurotransmitter release at inhibitory synapses.
27 Oct 2009 The presynaptic active zone is composed of a protein network that contains ELKS2alpha (a.k.a. CAST) as a central component. Here we demonstrate that in mice, deletion of ELKS2alpha caused a large increase in inhibitory, but not excitatory, ... Read more...
Latest indexed articles for 'Patch-Clamp Techniques'
These are the very latest articles for this heading:
- Rapid use-dependent down-regulation of gamma-aminobutyric acid type A receptors in rat mesencephalic trigeminal neurons. 30 Oct 2009
- Theta oscillations provide temporal windows for local circuit computation in the entorhinal-hippocampal loop. 27 Oct 2009
- ELKS2alpha/CAST deletion selectively increases neurotransmitter release at inhibitory synapses. 27 Oct 2009
- Evidence against AMPA receptor-lacking glutamatergic synapses in the superficial dorsal horn of the rat spinal cord. 19 Oct 2009
- Kv7/KCNQ channels control action potential phasing of pyramidal neurons during hippocampal gamma oscillations in vitro. 19 Oct 2009
- The time course of dopamine transmission in the ventral tegmental area. 19 Oct 2009
- In vivo role of a potassium channel-binding protein in regulating neuronal excitability and behavior. 19 Oct 2009
- Nitric oxide potentiation of locomotor activity in the spinal cord of the lamprey. 19 Oct 2009
- Protein kinase C mediates the synergistic interaction between agonists acting at alpha2-adrenergic and delta-opioid receptors in spinal cord. 19 Oct 2009
- Endocannabinoid-dependent homeostatic regulation of inhibitory synapses by miniature excitatory synaptic activities. 19 Oct 2009
- Mrgprd-expressing polymodal nociceptive neurons innervate most known classes of substantia gelatinosa neurons. 19 Oct 2009
- Grafting neural precursor cells promotes functional recovery in an SCA1 mouse model. 19 Oct 2009
- Functional recovery after lesion of a central pattern generator. 19 Oct 2009
- T-type calcium channel inhibition underlies the analgesic effects of the endogenous lipoamino acids. 19 Oct 2009
- Nonlinear integration of visual and haltere inputs in fly neck motor neurons. 19 Oct 2009
- Fast synaptic subcortical control of hippocampal circuits. 14 Oct 2009
- Bestrophin-1 encodes for the Ca2+-activated anion channel in hippocampal astrocytes. 12 Oct 2009
- Mechanisms underlying directional selectivity for frequency-modulated sweeps in the inferior colliculus revealed by in vivo whole-cell recordings. 12 Oct 2009
- KIF5B motor adaptor syntabulin maintains synaptic transmission in sympathetic neurons. 12 Oct 2009
- Cytoskeletal changes underlie estrogen's acute effects on synaptic transmission and plasticity. 12 Oct 2009
See a longer list of these articles.
Technical information about 'Patch-Clamp Techniques'
Definition: An electrophysiologic technique for studying cells, cell membranes, and occasionally isolated organelles. All patch-clamp methods rely on a very high-resistance seal between a micropipette and a membrane; the seal is usually attained by gentle suction. The four most common variants include on-cell patch, inside-out patch, outside-out patch, and whole-cell clamp. Patch-clamp methods are commonly used to voltage clamp, that is control the voltage across the membrane and measure current flow, but current-clamp methods, in which the current is controlled and the voltage is measured, are also used.
Descriptor UI: D018408
Alternative terms: Patch-Clamp Techniques; Patch Clamp Techniques; Patch-Clamp Technique; Technique, Patch-Clamp; Techniques, Patch-Clamp; Patch-Clamp Technics; Patch Clamp Technics; Patch-Clamp Technic; Technic, Patch-Clamp; Technics, Patch-Clamp; Whole-Cell Recording; Recording, Whole-Cell; Recordings, Whole-Cell; Whole Cell Recording; Whole-Cell Recordings; Voltage-Clamp Techniques; Technique, Voltage-Clamp; Techniques, Voltage-Clamp; Voltage Clamp Techniques; Voltage-Clamp Technique; Voltage-Clamp Technics; Technic, Voltage-Clamp; Technics, Voltage-Clamp; Voltage Clamp Technics; Voltage-Clamp Technic;
Allowable Qualifiers: classification; economics; history; instrumentation; methods; standards; trends; utilization; veterinary; statistics & numerical data; ethics;
Tree Number: E05.200.500.800;
History Note: 95
Technical Notes: a cytol technique for removing a tiny patch of plasma membrane; NIM