Plasmids
Research News and Information

Monday, November 23, 2009

Mechanisms of interactions of the nucleotide cofactor with the RepA protein of plasmid RSF1010. Binding dynamics studied using the fluorescence stopped-flow method.

8 Nov 2009 The dynamics of the nucleotide binding to a single, noninteracting nucleotide-binding site of the hexameric helicase RepA protein of plasmid RSF1010 has been examined, using the fluorescence stopped-flow method. The experiments have been performed ... Read more...

Quantification of radiation-induced single-strand breaks in plasmid DNA using a TUNEL/ELISA-based assay.

30 Oct 2009 To accurately quantify the number of single-strand breaks (SSBs) induced in plasmid DNA molecules after irradiation, a new type of assay methodology has been explored. The new method is based on the TUNEL (terminal deoxynucleotide transferase dUTP ... Read more...

Why the extended-spectrum beta-lactamases SHV-2 and SHV-5 are "hypersusceptible" to mechanism-based inhibitors.

18 Oct 2009 Extended-spectrum beta-lactamases (ESBLs) are derivatives of enzymes such as SHV-1 and TEM-1 that have undergone site-specific mutations that enable them to hydrolyze, and thus inactivate, oxyimino-cephalosporins, such as cefotaxime and ceftazidime. ... Read more...

Latest indexed articles for 'Plasmids'

These are the very latest articles for this heading:

• Mechanisms of interactions of the nucleotide cofactor with the RepA protein of plasmid RSF1010. Binding dynamics studied using the fluorescence stopped-flow method. 8 Nov 2009
• Quantification of radiation-induced single-strand breaks in plasmid DNA using a TUNEL/ELISA-based assay. 30 Oct 2009
• Why the extended-spectrum beta-lactamases SHV-2 and SHV-5 are "hypersusceptible" to mechanism-based inhibitors. 18 Oct 2009
• Regulation of phenylacetic acid degradation genes of Burkholderia cenocepacia K56-2. 16 Oct 2009
• Plasmid DNA production combining antibiotic-free selection, inducible high yield fermentation, and novel autolytic purification. 13 Oct 2009
• Enhanced radiosensitivity of SW480 cells via TRAIL up-regulation mediated by Egr-1 promoter. 29 Sep 2009
• Predominance of CTX-M-type extended-spectrum beta-lactamase genes among enterobacterial isolates from outpatients in Brazil. 29 Sep 2009
• Combination of IMP-4 metallo-beta-lactamase production and porin deficiency causes carbapenem resistance in a Klebsiella oxytoca clinical isolate. 29 Sep 2009
• Review of virulence factors of enterococcus: an emerging nosocomial pathogen. 29 Sep 2009
• Cyclooxygenase-2 Gly587Arg variant is associated with differential enzymatic activity and risk of esophageal squamous-cell carcinoma. 29 Sep 2009
• Cooperative interaction between protein inhibitor of activated signal transducer and activator of transcription-3 with epidermal growth factor receptor blockade in lung cancer. 29 Sep 2009
• Posttranslational regulation of Myc by promyelocytic leukemia zinc finger protein. 29 Sep 2009
• Metal ion interactions in the DNA cleavage/ligation active site of human topoisomerase IIalpha. 27 Sep 2009
• Differential utilization of T cell receptor TCRalpha/TCRdelta locus variable region gene segments is mediated by accessibility. 26 Sep 2009
• Identification of a bacteriocin and its cognate immunity factor expressed by Moraxella catarrhalis. 23 Sep 2009
• Association of Merkel cell polyomavirus-specific antibodies with Merkel cell carcinoma. 21 Sep 2009
• STING regulates intracellular DNA-mediated, type I interferon-dependent innate immunity. 21 Sep 2009
• Protein and DNA effectors control the TraI conjugative helicase of plasmid R1. 16 Sep 2009
• Plasmid r1 conjugative DNA processing is regulated at the coupling protein interface. 16 Sep 2009
• A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors. 16 Sep 2009

See a longer list of these articles.

Technical information about 'Plasmids'

Definition: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.

Descriptor UI: D010957

Alternative terms: Plasmids; Plasmid; Episomes; Episome;

Related Mesh Headings: Extrachromosomal Inheritance; Genetic Vectors;

Allowable Qualifiers: administration & dosage; adverse effects; analysis; antagonists & inhibitors; biosynthesis; blood; cerebrospinal fluid; chemical synthesis; classification; diagnostic use; drug effects; economics; genetics; history; immunology; isolation & purification; metabolism; pharmacokinetics; pharmacology; physiology; poisoning; radiation effects; secretion; standards; supply & distribution; therapeutic use; toxicity; ultrastructure; urine; chemistry; contraindications; agonists;

Tree Number: G05.360.600;

Online Note: use PLASMIDS to search EPISOMES 1975-77

History Note: 78(75); was see under EXTRACHROMOSOMAL INHERITANCE 1975-77; EPISOMES was see under EXTRACHROMOSOMAL INHERITANCE 1975-77

Technical Notes: GEN; prefer specifics