Polymerase Chain Reaction - Methods
Research News and Information

Sunday, November 22, 2009

Detection of epidermal growth factor receptor mutations in plasma by mutant-enriched PCR assay for prediction of the response to gefitinib in patients with non-small-cell lung cancer.

13 Nov 2009 The high frequency of epidermal growth factor receptor (EGFR) mutations in tyrosine kinase inhibitor-responsive non-small-cell lung cancer (NSCLC) cases is now well established, highlighting the predictive value of activating EGFR mutations in ... Read more...

Rapid molecular detection of Opisthorchis viverrini in human fecal samples by real-time polymerase chain reaction.

30 Oct 2009 Real-time fluorescence resonance energy transfer (FRET) polymerase chain reaction (PCR) supplemented with melting curve analysis is a highly sensitive and fast method offering a high throughput. We report the development of a real-time FRET PCR for ... Read more...

Malaria diagnosis by a polymerase chain reaction-based assay using a pooling strategy.

30 Oct 2009 Pooling clinical specimens reduces the number of assays needed when screening for infectious diseases. Polymerase chain reaction (PCR)-based assays are the most sensitive tests to diagnose malaria, but its high cost limits its use. We adapted a ... Read more...

Latest indexed articles for 'Polymerase Chain Reaction - Methods'

These are the very latest articles for this heading:

• Detection of epidermal growth factor receptor mutations in plasma by mutant-enriched PCR assay for prediction of the response to gefitinib in patients with non-small-cell lung cancer. 13 Nov 2009
• Rapid molecular detection of Opisthorchis viverrini in human fecal samples by real-time polymerase chain reaction. 30 Oct 2009
• Malaria diagnosis by a polymerase chain reaction-based assay using a pooling strategy. 30 Oct 2009
• Assessment of the low-cost Cavidi ExaVir Load assay for monitoring HIV viral load in pediatric and adult patients. 30 Oct 2009
• Clinical performance of JAK2 V617F mutation detection assays in a molecular diagnostics laboratory: evaluation of screening and quantitation methods. 30 Oct 2009
• Impact of genetic polymorphisms in cytomegalovirus glycoprotein B on outcomes in solid-organ transplant recipients with cytomegalovirus disease. 13 Oct 2009
• Prenatal diagnosis of hemoglobin Bart's hydrops fetalis with gap-PCR system. 5 Oct 2009
• Gene copy number: learning to count past two. 29 Sep 2009
• Reply to: "Experimental aspects of copy number variant assays at CCL3L1". 29 Sep 2009
• Comparison between pp65 antigenemia assay and quantitative real-time polymerase chain reaction for detection of active cytomegalovirus infection in routine diagnostics. 29 Sep 2009
• The Staphylococcus aureus lineage-specific markers collagen adhesin and toxic shock syndrome toxin 1 distinguish multilocus sequence typing clonal complexes within spa clonal complexes. 29 Sep 2009
• Genetic profiles of Shiga toxin and intimin genes found in stool broth cultures: a 2-year reference laboratory study. 29 Sep 2009
• Laboratory diagnosis of infections due to blood and tissue parasites. 29 Sep 2009
• Acute hepatitis E virus infection in Taiwan 2002-2006 revisited: PCR shows frequent co-infection with multiple hepatitis viruses. 29 Sep 2009
• Detection of congenital cytomegalovirus infection using umbilical cord blood samples in a screening survey. 29 Sep 2009
• Human papillomavirus DNA detected in peripheral blood samples from healthy Australian male blood donors. 29 Sep 2009
• Comparison of human papillomavirus genotyping using commercial assays based on PCR and reverse hybridization methods. 29 Sep 2009
• MALS: an efficient strategy for multiple site-directed mutagenesis employing a combination of DNA amplification, ligation and suppression PCR. 22 Sep 2009
• Syphilitic panuveitis with retinal necrosis in an HIV positive man confirmed by Treponema pallidum PCR. 15 Sep 2009
• Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo. 13 Sep 2009

See a longer list of these articles.

Technical information about 'Polymerase Chain Reaction'

Definition: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.

Descriptor UI: D016133

Alternative terms: Polymerase Chain Reaction; Polymerase Chain Reactions; Reaction, Polymerase Chain; Reactions, Polymerase Chain; PCR; Inverse PCR; PCR, Inverse; Inverse Polymerase Chain Reaction; Nested Polymerase Chain Reaction; Nested PCR; PCR, Nested; Anchored PCR; PCR, Anchored; Anchored Polymerase Chain Reaction;

Related Mesh Headings: Sequence Tagged Sites; Ligase Chain Reaction;

Allowable Qualifiers: classification; economics; history; instrumentation; methods; mortality; standards; trends; utilization; veterinary; statistics & numerical data; contraindications; ethics; drug effects; radiation effects;

Tree Number: E05.393.620.500;

History Note: 91(90); was see under GENE AMPLIFICATION 1990