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Polymerase Chain Reaction - Standards
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Definition of 'Polymerase Chain Reaction'In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. |
Monday, November 23, 2009
30 Aug 2009 
The comparison between the Vitek Immunodiagnostic Assay System (VIDAS) and PCR methods for the detection of the pathogenic microorganisms Salmonella Typhimurium, Listeria monocytogenes, Escherichia coli O157:H7, Clostridium perfringens, and ... Read more...
10 Aug 2009 The virus-resistant papaya (Carica papaya L.), Huanong no. 1, was the genetically modified (GM) fruit approved for growing in China in 2006. To implement the labeling regulation of GM papaya and its derivates, the development of papaya endogenous ... Read more...
Selection of reference genes for expression studies with fish myogenic cell cultures.
8 Aug 2009 BACKGROUND: Relatively few studies have used cell culture systems to investigate gene expression and the regulation of myogenesis in fish. To produce robust data from quantitative real-time PCR mRNA levels need to be normalised using internal ... Read more...
Latest indexed articles for 'Polymerase Chain Reaction - Standards'
These are the very latest articles for this heading:
- Gene copy number: learning to count past two.
29 Sep 2009 - Does HUMARA assay for assessment of clonal hematopoiesis have shortcomings? 8 Sep 2009
- Comparison between the Vitek Immunodiagnostic Assay System and PCR for the detection of pathogenic microorganisms in an experimental dry sausage during its curing process. 30 Aug 2009
- Applicability of the chymopapain gene used as endogenous reference gene for transgenic huanong no. 1 papaya detection. 10 Aug 2009
- Selection of reference genes for expression studies with fish myogenic cell cultures. 8 Aug 2009
- Development of a Real-Time PCR assay for the specific detection of Brochothrix thermosphacta in fresh and spoiled raw meat. Aug 2009
- Evaluation of polymerase chain reaction on amniotic fluid for diagnosis of congenital toxoplasmosis. 30 Jul 2009
- Limiting false-positive polymerase chain reaction results: detection of DNA and mRNA to differentiate viable from dead bacteria. 30 Jul 2009
- Development of primers for detection of heat-treated cetacean materials in porcine meat and bone meal. 29 Jun 2009
- Specific PCR detection of Arcobacter butzleri, Arcobacter cryaerophilus, Arcobacter skirrowii, and Arcobacter cibarius in chicken meat. 29 Jun 2009
- A real-time PCR assay to identify and discriminate among wild-type and vaccine strains of varicella-zoster virus and herpes simplex virus in clinical specimens, and comparison with the clinical diagnoses. 29 Jun 2009
- Effect of storage regime on the stability of DNA used as a calibration standard for real-time polymerase chain reaction. 19 Jun 2009
- Reference genes for gene expression studies on non-small cell lung cancer.
16 Jun 2009 - PCR-restriction fragment length polymorphism analysis as a tool for Mycobacterium species identification in lepromas for lepromin production. 30 May 2009
- Design of FRET-TaqMan probes for multiplex real-time PCR using an internal positive control. 30 May 2009
- Quantitative detection of Plasmodium falciparum DNA in saliva, blood, and urine. 30 May 2009
- Comprehensive assessment of the TCRBV repertoire in small T-cell samples by means of an improved and convenient multiplex PCR method. 30 May 2009
- Effects of quantum dots in polymerase chain reaction. 26 May 2009
- Kinetics of hepatitis A virus replication in vivo and in vitro using negative-strand quantitative PCR. 25 May 2009
- Characterization and interlaboratory comparison of a gene expression signature for differentiating genotoxic mechanisms. 20 May 2009
See a longer list of these articles.
Technical information about 'Polymerase Chain Reaction'
Definition: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Descriptor UI: D016133
Alternative terms: Polymerase Chain Reaction; Polymerase Chain Reactions; Reaction, Polymerase Chain; Reactions, Polymerase Chain; PCR; Inverse PCR; PCR, Inverse; Inverse Polymerase Chain Reaction; Nested Polymerase Chain Reaction; Nested PCR; PCR, Nested; Anchored PCR; PCR, Anchored; Anchored Polymerase Chain Reaction;
Related Mesh Headings: Sequence Tagged Sites; Ligase Chain Reaction;
Allowable Qualifiers: classification; economics; history; instrumentation; methods; mortality; standards; trends; utilization; veterinary; statistics & numerical data; contraindications; ethics; drug effects; radiation effects;
Tree Number: E05.393.620.500;
History Note: 91(90); was see under GENE AMPLIFICATION 1990