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Polymerase Chain Reaction - Veterinary
Research News and Information
Definition of 'Polymerase Chain Reaction'In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. |
Monday, November 23, 2009
29 Sep 2009 
Anti-Salmonella spp. egg yolk antibodies (IgY) simultaneously directed against Salmonella Enteritidis and Salmonella Typhimurium were tested to determine if their inclusion in feed decreased Salmonella spp. cecal colonization in experimentally ... Read more...
30 Aug 2009 The aim of this investigation was to determine the presence of the opportunistic pathogen Clostridium perfringens by PCR and DNA sequencing, without previous cultivation. This methodology was then used to investigate how C. perfringens was affected ... Read more...
Persistent infection of turkeys with an avirulent strain of turkey hemorrhagic enteritis virus.
30 Aug 2009 The Virginia avirulent strain (VAS) of turkey hemorrhagic enteritis virus (THEV), which is commonly used in live vaccines for commercial turkeys, was studied to determine characteristics of infection. It has been observed that turkeys infected with ... Read more...
Latest indexed articles for 'Polymerase Chain Reaction - Veterinary'
These are the very latest articles for this heading:
- Effects of feed supplementation with specific hen egg yolk antibody (immunoglobin Y) on Salmonella species cecal colonization and growth performances of challenged broiler chickens. 29 Sep 2009
- Evaluation of PCR and DNA sequencing for direct detection of Clostridium perfringens in the intestinal tract of broilers. 30 Aug 2009
- Persistent infection of turkeys with an avirulent strain of turkey hemorrhagic enteritis virus. 30 Aug 2009
- Identification of group I-III avian adenovirus by PCR coupled with direct sequencing of the hexon gene. 30 Aug 2009
- Development of quantitative real-time polymerase chain reaction for detection of and discrimination between Erysipelothrix rhusiopathiae and other Erysipelothrix species. 30 Aug 2009
- A first case of ehrlichiosis in a horse in Poland. 30 Aug 2009
- PCR detection of ranavirus in adult anurans from the Louisville Zoological Garden. 30 Aug 2009
- Infection with Mycobacterium avium subspecies avium in a 10 year old Freiberger mare. 30 Aug 2009
- [Prevalence of Tritrichomonas foetus among Dutch cats] 30 Aug 2009
- Evaluation of field and laboratory protocols used to detect avian influenza viruses in wild aquatic birds. 30 Aug 2009
- Flock-level prevalence of Clostridium colinum in broiler flocks with digestive disease in Jordan by polymerase chain reaction. 30 Jul 2009
- Gene expression profiles of two B-complex disparate, genetically inbred Fayoumi chicken lines that differ in susceptibility to Eimeria maxima. 30 Jul 2009
- Detection of bloodstream infection in neonatal foals with suspected sepsis using real-time PCR.
23 Jul 2009 - Infertility and venereal disease in cattle inseminated with semen containing bovine herpesvirus type 5. 23 Jul 2009
- Toxoplasma gondii: Evidence for the transmission by semen in dogs. 17 Jul 2009
- IS711-based real-time PCR assay as a tool for detection of Brucella spp. in wild boars and comparison with bacterial isolation and serology. 12 Jul 2009
- Australian surveillance for avian influenza viruses in wild birds between July 2005 and June 2007. 29 Jun 2009
- Faecal shedding and serological cross-sectional study of Lawsonia intracellularis in horses in the state of Minas Gerais, Brazil. 29 Jun 2009
- [Anaplasma phagocytophilum infection in a cat in Switzerland] 29 Jun 2009
- Technical note: use of transfer RNA-intergenic spacer PCR combined with capillary electrophoresis to identify coagulase-negative Staphylococcus species originating from bovine milk and teat apices. 29 Jun 2009
See a longer list of these articles.
Technical information about 'Polymerase Chain Reaction'
Definition: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Descriptor UI: D016133
Alternative terms: Polymerase Chain Reaction; Polymerase Chain Reactions; Reaction, Polymerase Chain; Reactions, Polymerase Chain; PCR; Inverse PCR; PCR, Inverse; Inverse Polymerase Chain Reaction; Nested Polymerase Chain Reaction; Nested PCR; PCR, Nested; Anchored PCR; PCR, Anchored; Anchored Polymerase Chain Reaction;
Related Mesh Headings: Sequence Tagged Sites; Ligase Chain Reaction;
Allowable Qualifiers: classification; economics; history; instrumentation; methods; mortality; standards; trends; utilization; veterinary; statistics & numerical data; contraindications; ethics; drug effects; radiation effects;
Tree Number: E05.393.620.500;
History Note: 91(90); was see under GENE AMPLIFICATION 1990