Polymerase Chain Reaction
Research News and Information

Sunday, November 08, 2009

Association of HPV16 E6 variants with diagnostic severity in cervical cytology samples of 354 women in a US population.

29 Nov 2009 It has been suggested that DNA sequence variants of HPV16 contribute to differences in the behavior of individual cervical lesions. To address this question, we have analyzed the association of HPV16 variants with diagnostic severity in 354 ... Read more...

Carbonic anhydrase IX and human papillomavirus as diagnostic biomarkers of cervical dysplasia/neoplasia in women with a cytologic diagnosis of atypical glandular cells: a Gynecologic Oncology Group study in United States.

13 Nov 2009 High-risk human papillomavirus (H-HPV) infection is strongly linked to cervical neoplasia, but its role in detecting glandular lesions (GLs) is unclear. In the cervix, carbonic anhydrase IX (CA-IX) is expressed in cervical neoplasia, but rarely in ... Read more...

The potential impact of human papillomavirus vaccination in contemporary cytologically screened populations may be underestimated: an observational retrospective analysis of invasive cervical cancers.

13 Nov 2009 The aim of this study was to determine the proportion of invasive cervical cancers attributable to human papillomavirus (HPV) types 16 and 18 in a contemporary, cytologically well-screened UK population. This was achieved in a retrospective ... Read more...

Latest indexed articles for 'Polymerase Chain Reaction'

These are the very latest articles for this heading:

• Association of HPV16 E6 variants with diagnostic severity in cervical cytology samples of 354 women in a US population. 29 Nov 2009
• Carbonic anhydrase IX and human papillomavirus as diagnostic biomarkers of cervical dysplasia/neoplasia in women with a cytologic diagnosis of atypical glandular cells: a Gynecologic Oncology Group study in United States. 13 Nov 2009
• The potential impact of human papillomavirus vaccination in contemporary cytologically screened populations may be underestimated: an observational retrospective analysis of invasive cervical cancers. 13 Nov 2009
• Absence of IDH2 codon 172 mutation in common human cancers. 13 Nov 2009
• Detection of epidermal growth factor receptor mutations in plasma by mutant-enriched PCR assay for prediction of the response to gefitinib in patients with non-small-cell lung cancer. 13 Nov 2009
• Repopulation of vascularized bone allotransplants with recipient-derived cells: detection by laser capture microdissection and real-time PCR. 30 Oct 2009
• Blood infection diagnosis by 16S rDNA broad-spectrum polymerase chain reaction: the relationship between antibiotic treatment and bacterial DNA load. 30 Oct 2009
• Dendritic cell transfected with secondary lymphoid-tissue chemokine and/or interleukin-2 gene-enhanced cytotoxicity of T-lymphocyte in human bladder tumor cell S in vitro. 30 Oct 2009
• Upregulated p27kip1 can downregulate survivin expression and inhibit telomerase activity in gastric carcinoma cells. 30 Oct 2009
• Epigenetic regulation of Myc on retinoic acid receptor beta and PDLIM4 in RWPE1 cells. 30 Oct 2009
• Consistent genomic alterations in carcinoma in situ of the urinary bladder confirm the presence of two major pathways in bladder cancer development. 30 Oct 2009
• Anopheles gambiae alkaline phosphatase is a functional receptor of Bacillus thuringiensis jegathesan Cry11Ba toxin. 18 Oct 2009
• Recommendations for diagnosis of shiga toxin--producing Escherichia coli infections by clinical laboratories. 14 Oct 2009
• The association between -1304T>G polymorphism in the promoter of MKK4 gene and the risk of sporadic colorectal cancer in southern Chinese population. 13 Oct 2009
• Melanocortin receptor 1 variants and melanoma risk: a study of 2 European populations. 13 Oct 2009
• Association of HLA-DRB1, interleukin-6 and cyclin D1 polymorphisms with cervical cancer in the Swedish population--a candidate gene approach. 13 Oct 2009
• TGFbetaR2 aberrant methylation is a potential prognostic marker and therapeutic target in multiple myeloma. 13 Oct 2009
• Glucocorticoid regulation of the circadian clock modulates glucose homeostasis. 3 Oct 2009
• Bacterial coinfections in lung tissue specimens from fatal cases of 2009 pandemic influenza A (H1N1) - United States, May-August 2009. 30 Sep 2009
• Predominance of CTX-M-type extended-spectrum beta-lactamase genes among enterobacterial isolates from outpatients in Brazil. 29 Sep 2009

See a longer list of these articles.

Technical information about 'Polymerase Chain Reaction'

Definition: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.

Descriptor UI: D016133

Alternative terms: Polymerase Chain Reaction; Polymerase Chain Reactions; Reaction, Polymerase Chain; Reactions, Polymerase Chain; PCR; Inverse PCR; PCR, Inverse; Inverse Polymerase Chain Reaction; Nested Polymerase Chain Reaction; Nested PCR; PCR, Nested; Anchored PCR; PCR, Anchored; Anchored Polymerase Chain Reaction;

Related Mesh Headings: Sequence Tagged Sites; Ligase Chain Reaction;

Allowable Qualifiers: classification; economics; history; instrumentation; methods; mortality; standards; trends; utilization; veterinary; statistics & numerical data; contraindications; ethics; drug effects; radiation effects;

Tree Number: E05.393.620.500;

History Note: 91(90); was see under GENE AMPLIFICATION 1990