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Polymerase Chain Reaction
Research News and Information
Definition of 'Polymerase Chain Reaction'In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. |
Saturday, November 21, 2009
29 Nov 2009 
It has been suggested that DNA sequence variants of HPV16 contribute to differences in the behavior of individual cervical lesions. To address this question, we have analyzed the association of HPV16 variants with diagnostic severity in 354 ... Read more...
15 Nov 2009 Protein synthesis is a highly conserved process in all living cells involving several members of the translation factor (TRAFAC) class of P-loop GTPases, which play essential roles during translation. The universally conserved GTPase HflX has ... Read more...
13 Nov 2009 INTRODUCTION: Light microscopy examination of blood slides is the main method of detecting malaria infection; however, it has limited sensitivity. Low-density infections are most likely to be missed, but they contribute to the infectious reservoir. ... Read more...
Latest indexed articles for 'Polymerase Chain Reaction'
These are the very latest articles for this heading:
- Association of HPV16 E6 variants with diagnostic severity in cervical cytology samples of 354 women in a US population. 29 Nov 2009
- Toward understanding the function of the universally conserved GTPase HflX from Escherichia coli: a kinetic approach. 15 Nov 2009
- Submicroscopic infection in Plasmodium falciparum-endemic populations: a systematic review and meta-analysis. 13 Nov 2009
- Carbonic anhydrase IX and human papillomavirus as diagnostic biomarkers of cervical dysplasia/neoplasia in women with a cytologic diagnosis of atypical glandular cells: a Gynecologic Oncology Group study in United States. 13 Nov 2009
- The potential impact of human papillomavirus vaccination in contemporary cytologically screened populations may be underestimated: an observational retrospective analysis of invasive cervical cancers. 13 Nov 2009
- Absence of IDH2 codon 172 mutation in common human cancers.
13 Nov 2009 - Detection of epidermal growth factor receptor mutations in plasma by mutant-enriched PCR assay for prediction of the response to gefitinib in patients with non-small-cell lung cancer. 13 Nov 2009
- Requirement of a unique Ca(2+)-binding loop for folding of Tk-subtilisin from a hyperthermophilic archaeon. 8 Nov 2009
- Mutational hot spot potential of a novel base pair mutation of the CSPG2 gene in a family with Wagner syndrome. 30 Oct 2009
- Common CCR 5 polymorphism in stroke: the CCR 5 delta32 polymorphism differentiates cardioembolism from other aetiologies of ischaemic cerebrovascular diseases. 30 Oct 2009
- New alternative vaccine component against mycobacterium tuberculosis--heat shock protein 16.3 or its T-cell epitope. 30 Oct 2009
- Rapid molecular detection of Opisthorchis viverrini in human fecal samples by real-time polymerase chain reaction. 30 Oct 2009
- Malaria diagnosis by a polymerase chain reaction-based assay using a pooling strategy. 30 Oct 2009
- African tick bite Fever in a Taiwanese traveler returning from South Africa: molecular and serologic studies. 30 Oct 2009
- Assessment of the low-cost Cavidi ExaVir Load assay for monitoring HIV viral load in pediatric and adult patients. 30 Oct 2009
- T-cell receptor gene rearrangement and CD30 immunoreactivity in traumatic ulcerative granuloma with stromal eosinophilia of the oral cavity. 30 Oct 2009
- Clinical performance of JAK2 V617F mutation detection assays in a molecular diagnostics laboratory: evaluation of screening and quantitation methods. 30 Oct 2009
- Relative quantity of cerebrospinal fluid herpes simplex virus DNA in adult cases of encephalitis and meningitis. 30 Oct 2009
- The small heat shock protein Hsp27 protects cortical neurons against the toxic effects of beta-amyloid peptide. 30 Oct 2009
- Phospholipid transfer protein reduces phosphorylation of tau in human neuronal cells. 30 Oct 2009
See a longer list of these articles.
Technical information about 'Polymerase Chain Reaction'
Definition: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Descriptor UI: D016133
Alternative terms: Polymerase Chain Reaction; Polymerase Chain Reactions; Reaction, Polymerase Chain; Reactions, Polymerase Chain; PCR; Inverse PCR; PCR, Inverse; Inverse Polymerase Chain Reaction; Nested Polymerase Chain Reaction; Nested PCR; PCR, Nested; Anchored PCR; PCR, Anchored; Anchored Polymerase Chain Reaction;
Related Mesh Headings: Sequence Tagged Sites; Ligase Chain Reaction;
Allowable Qualifiers: classification; economics; history; instrumentation; methods; mortality; standards; trends; utilization; veterinary; statistics & numerical data; contraindications; ethics; drug effects; radiation effects;
Tree Number: E05.393.620.500;
History Note: 91(90); was see under GENE AMPLIFICATION 1990