Transfection - Methods
Research News and Information

Monday, November 23, 2009

A comparative study of transfection methods for RNA interference in bone marrow-derived murine dendritic cells.

30 Oct 2009 Selective gene silencing using RNA interference (RNAi) has been shown to be an efficient method for manipulation of cellular functions. In this study, we compare three previously established methods for transfection of murine bone marrow-derived DC ... Read more...

Dual palmitoylation of NR2 subunits regulates NMDA receptor trafficking.

27 Oct 2009 Modification of NMDA receptor function and trafficking contributes to the regulation of synaptic transmission and is important for several forms of synaptic plasticity. Here, we report that NMDA receptor subunits NR2A and NR2B have two distinct ... Read more...

F-BAR proteins of the syndapin family shape the plasma membrane and are crucial for neuromorphogenesis.

19 Oct 2009 Coordinated functions of the actin cytoskeleton and microtubules, which require careful control in time and space, are indispensable for the drastic alterations of neuronal morphology during neuromorphogenesis and neuronal network formation. Actin ... Read more...

Latest indexed articles for 'Transfection - Methods'

These are the very latest articles for this heading:

• A comparative study of transfection methods for RNA interference in bone marrow-derived murine dendritic cells. 30 Oct 2009
• Dual palmitoylation of NR2 subunits regulates NMDA receptor trafficking. 27 Oct 2009
• F-BAR proteins of the syndapin family shape the plasma membrane and are crucial for neuromorphogenesis. 19 Oct 2009
• The TC10-Exo70 complex is essential for membrane expansion and axonal specification in developing neurons. 19 Oct 2009
• Disruption of the axon initial segment cytoskeleton is a new mechanism for neuronal injury. 19 Oct 2009
• Bestrophin-1 encodes for the Ca2+-activated anion channel in hippocampal astrocytes. 12 Oct 2009
• KIF5B motor adaptor syntabulin maintains synaptic transmission in sympathetic neurons. 12 Oct 2009
• Activated actin-depolymerizing factor/cofilin sequesters phosphorylated microtubule-associated protein during the assembly of alzheimer-like neuritic cytoskeletal striations. 12 Oct 2009
• Retrograde viral vector-mediated inhibition of pontospinal noradrenergic neurons causes hyperalgesia in rats. 12 Oct 2009
• Distinct domains within PSD-95 mediate synaptic incorporation, stabilization, and activity-dependent trafficking. 12 Oct 2009
• Lentivirus production. 30 Sep 2009
• Glutamate agonist LY404,039 for treating schizophrenia has affinity for the dopamine D2(High) receptor. 29 Sep 2009
• The dopaminergic stabilizer ASP2314/ACR16 selectively interacts with D2(High) receptors. 29 Sep 2009
• Dynamics of fast and slow inhibition from cerebellar golgi cells allow flexible control of synaptic integration. 22 Sep 2009
• Roles of disrupted-in-schizophrenia 1-interacting protein girdin in postnatal development of the dentate gyrus. 22 Sep 2009
• DISC1 regulates new neuron development in the adult brain via modulation of AKT-mTOR signaling through KIAA1212. 22 Sep 2009
• Characterization of CLP36/Elfin/PDLIM1 in the nervous system. 21 Sep 2009
• A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors. 16 Sep 2009
• Identification of novel spartin-interactors shows spartin is a multifunctional protein. 14 Sep 2009
• TDP-43 is recruited to stress granules in conditions of oxidative insult. 14 Sep 2009

See a longer list of these articles.

Technical information about 'Transfection'

Definition: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.

Descriptor UI: D014162

Alternative terms: Transfection; Transfections;

Related Mesh Headings: Transformation, Bacterial; Transformation, Genetic; Electroporation;

Allowable Qualifiers: classification; economics; history; instrumentation; methods; standards; trends; utilization; veterinary; statistics & numerical data; ethics;

Tree Number: E05.393.350.810; G05.355.760.860;

History Note: 78(75); was see under TRANSFORMATION, GENETIC 1975-77

Technical Notes: coordinate with recipient/genet if permitted + specific donor/genet; do not confuse with genetic transvection, an epigenetic phenomenon (index under EPIGENESIS, GENETIC)