Research article summary (published 29 Sep 1999):

31P and 1H NMR spectroscopic studies of liver extracts of carbon tetrachloride-treated rats.

Full Abstract

NMR spectroscopy was used to examine hepatic metabolism in cirrhosis with a particular focus on markers of functional cellular hypoxia. (31)P and (1)H NMR spectra were obtained from liver extracts from control rats and from rats with carbon tetrachloride-induced cirrhosis. A decrease of 34% in total phosphorus content was observed in cirrhotic rats, parallelling a reduction of 40% in hepatocyte mass as determined by morphometric analysis. Hypoxia appeared to be present in cirrhotic rats, as evidenced by increased inorganic phosphate levels, decreased ATP levels, decreased ATP:ADP ratios (1.72 +/- 0.40 vs 2.48 +/- 0.50, p < 0.01), and increased inorganic phosphate:ATP ratios (2.77 +/- 0.48 vs 1.62 +/- 0.24, p < 0.00001). When expressed as a percentage of the total phosphorus content, higher levels of phosphoethanolamine and lower levels of NAD and glycerophosphoethanolamine were detected in cirrhotic rats. Cirrhotic rats also had increased phosphomonoester:phosphodiester ratios (5.73 +/- 2.88 vs 2.53 +/- 0.52, p < 0.01). These findings are indicative of extensive changes in cellular metabolism in the cirrhotic liver, with many findings attributable to the presence of intracellular hypoxia. Copyright 1999 John Wiley & Sons, Ltd.

Author information

Author/s: Harvey, P J (PJ); Gready, J E (JE); Hickey, H M (HM); Le Couteur, D G (DG); McLean, A J (AJ);

Affiliation: Division of Biochemistry and Molecular Biology, John Curtin School of Medical Research, Australian National University, ACT 0200, Australia. Peta.Harvey(-atsign-)anu.edu.au

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't

Journal: NMR in biomedicine (NMR Biomed), published in ENGLAND. (Language: eng)

Reference: 1999-Oct; vol 12 (issue 6) : pp 395-401

Dates: Created 1999/12/22; Completed 1999/12/22; Revised 2006/11/15;

PMID: 10516622, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adenosine Diphosphate - metabolism Adenosine Triphosphate - metabolism Animals Carbon Tetrachloride - toxicity Carbon Tetrachloride Poisoning - metabolism Cell Hypoxia Disease Models, Animal Liver - chemistry; metabolism

Liver Cirrhosis, Experimental - chemically induced; metabolism Male Nuclear Magnetic Resonance, Biomolecular Phosphorus Protons Rats Rats, Wistar Tissue Extracts - analysis

Associated Chemicals: Protons (0) ; Tissue Extracts (0) ; Carbon Tetrachloride (56-23-5) ; Adenosine Triphosphate (56-65-5) ; Adenosine Diphosphate (58-64-0) ; Phosphorus (7723-14-0)

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