Research article summary (published 11 Dec 1999):

Localization of alternatively spliced NMDAR1 glutamate receptor isoforms in rat striatal neurons.

Full Abstract

Alternative splicing of the mRNA encoding the N-methyl-D-aspartate (NMDA) receptor subunit NR1 changes the structural, physiologic, and pharmacologic properties of the resultant NMDA receptors. We used dual label immunocytochemistry and confocal microscopy to localize the four alternatively spliced segments of the NR1 subunit (N1, C1, C2, and C2') in rat striatal neurons. Striatofugal projection neurons and four populations of interneurons were studied. Projection neurons, which were identified by immunolabeling for calbindin and by retrograde tracing from the globus pallidus and the substantia nigra, were the only striatal neurons containing C1 segment immunoreactivity. Projection neurons were also C2 segment immunopositive, as were all other neuronal populations studied. Projection neurons were C2' segment immunonegative. In contrast, each of the interneuron types were labeled by the antibody to the C2' segment: nitric oxide synthase interneurons were labeled intensely, calretinin and parvalbumin neurons were labeled moderately strongly, and cholinergic neurons were also labeled but less strongly than the other types of interneurons. Parvalbumin interneurons showed distinct N1 segment immunolabeling, which was not found in other types of striatal neurons. Our results suggest that all striatal neurons studied synthesize NR1 subunit proteins, but the isoforms of the protein present in projection neurons and the several types of interneurons are distinct. This differential expression of NR1 isoforms may affect both neuronal function and selective vulnerability of neurons to injury. Copyright 1999 Wiley-Liss, Inc.

Author information

Author/s: Küppenbender, K D (KD); Albers, D S (DS); Iadarola, M J (MJ); Landwehrmeyer, G B (GB); Standaert, D G (DG);

Affiliation: Department of Neurology, Albert-Ludwigs-Universität, D-79106 Freiburg, Germany.

Grants: NS31579 (Agency:NINDS NIH HHS) ; NS34361 (Agency:NINDS NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.

Journal: The Journal of comparative neurology (J Comp Neurol), published in UNITED STATES. (Language: eng)

Reference: 1999-Dec; vol 415 (issue 2) : pp 204-17

Dates: Created 1999/12/10; Completed 1999/12/10; Revised 2007/11/14;

PMID: 10545160, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Alternative Splicing Animals Calcium-Binding Protein, Vitamin D-Dependent - analysis Choline O-Acetyltransferase - analysis Immunohistochemistry Male Neostriatum - chemistry; cytology; physiology Neural Pathways - cytology

Neurons - chemistry; cytology; physiology Parvalbumins - analysis Protein Isoforms - genetics RNA, Messenger - analysis Rats Rats, Sprague-Dawley Receptors, Glutamate - genetics Receptors, N-Methyl-D-Aspartate - genetics

Associated Chemicals: Calcium-Binding Protein, Vitamin D-Dependent (0) ; NMDA receptor A1 (0) ; Parvalbumins (0) ; Protein Isoforms (0) ; RNA, Messenger (0) ; Receptors, Glutamate (0) ; Receptors, N-Methyl-D-Aspartate (0) ; calbindin (0) ; calretinin (0) ; Choline O-Acetyltransferase (EC 2.3.1.6)

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