Research article summary (published 30 May 2000):

Urine hyperglycosylated hCG plus ultrasound biometry for detection of down syndrome in the second trimester in a high-risk population.

Full Abstract

OBJECTIVE: To evaluate measurement of levels of urine hyperglycosylated hCG, a form of hCG with abnormally branched oligosaccharide side chains, in conjunction with ultrasound biometry for Down syndrome risk prediction in an at-risk group. METHOD: We prospectively measured urine hyperglycosylated hCG levels, humeral length, and nuchal thickness in women who had second-trimester amniocentesis. Urine hyperglycosylated hCG levels were measured by a two-step enzyme-immunometric assay using monoclonal antibody beta152. Humeral length, nuchal thickness, and hyperglycosylated hCG values were expressed as multiples of the median, and the Down syndrome screening efficiency of the three analytes plus age was determined. A receiver operating characteristic (ROC) curve was generated, and the area under the curve was used to assess the Down syndrome screening performance of the algorithm. RESULTS: There were 23 cases of Down syndrome among 1016 singleton pregnancies. Mean gestational age (+/- standard deviation) was 16.1 +/- 1.2 weeks at the time of amniocentesis. Mean maternal age was 37.1 +/- 3.2 years. Biometry and measurement of hyperglycosylated hCG levels had a 91.3% detection rate at a 3.2% false-positive rate and a 100% detection rate at a 10.7% false-positive rate. The area under the ROC curve was 0.986 (P <.001), and that for measurement of hyperglycosylated hCG levels plus age was 0.941 (P <.001). The area under the curve was significantly larger with combined biochemical and biometry markers compared with measurement of hyperglycosylated hCG levels plus age alone (P <.02), proving that the former was superior to the latter. CONCLUSION: A new Down syndrome biochemical marker combined with ultrasound biometry had a high screening efficiency in a high-risk group. All cases of Down syndrome in this study population would have been detected at an amniocentesis rate of less than 10.7%. Our results appear superior to those found with other second-trimester algorithms. The combination is promising as an alternative to "automatic" genetic amniocentesis in women of advanced maternal age and other high-risk groups.

Author information

Author/s: Bahado-Singh, R (R); Oz, U (U); Shahabi, S (S); Omrani, A (A); Mahoney, M (M); Cole, L (L);

Affiliation: Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, Connecticut, USA. ray.bahado-sing(-atsign-)yale.edu

Journal and publication information

Publication Type: Journal Article

Journal: Obstetrics and gynecology (Obstet Gynecol), published in UNITED STATES. (Language: eng)

Reference: 2000-Jun; vol 95 (issue 6 Pt 1) : pp 889-94

Dates: Created 2000/07/19; Completed 2000/07/19; Revised 2009/10/26;

PMID: 10831986, status: MEDLINE (last retrieval date: 10/26/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adult Biological Markers Biometry - methods Chorionic Gonadotropin - urine Down Syndrome - diagnosis Female Glycosylation Humans

Humans Middle Aged Pregnancy Pregnancy Trimester, Second Prenatal Diagnosis Prospective Studies ROC Curve Ultrasonography, Prenatal

Associated Chemicals: Biological Markers (0) ; Chorionic Gonadotropin (0)

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