Research article summary (published 30 May 2000):

Simultaneous determination of human plasma levels of four selective serotonin reuptake inhibitors by high-performance liquid chromatography.

Full Abstract

A reversed-phase high-performance liquid chromatography (HPLC) method with fluorimetric detection, which allows the simultaneous determination of plasma concentrations of four selective serotonin reuptake inhibitors (SSRIs) is presented. Fluvoxamine, paroxetine, sertraline, and fluoxetine were extracted from plasma with ethyl acetate and then derivatized with dansyl chloride. The analytes were separated using Hypersyl ODS C18 (5 microm) 250 x 4.6 mm column (ThermoQuest, Runcorn, UK). For continuous gradient separation, the mobile phase consists of two eluents, acetonitrile and potassium phosphate buffer (10 mmol/L, pH 7.2) at total flow rate of 1.5 mL/min. Detection was carried out at lambda exc = 366 nm and lambda em = 490 nm. The authors found recoveries of 90% to 95% for fluvoxamine, 94% to 100% for paroxetine, 88% to 95% for sertraline, 93% to 100% for fluoxetine, and 97% to 100% for internal standard (nortriptyline). Imprecision of the method ranged from 2.5% to 8.9%. The assay was linear from 10 to 1500 ng/mL for sertraline, and from 5 to 1500 ng/mL for the other drugs. The authors conclude that this method is suitable for monitoring antidepressant therapy. In addition, the authors report the effects of adding paroxetine to fluvoxamine on plasma levels in a group of patients in combined drug therapy.

Author information

Author/s: Lucca, A (A); Gentilini, G (G); Lopez-Silva, S (S); Soldarini, A (A);

Affiliation: Istituto Scientifico Ospedale San Raffaele, Department of Neuropsychiatric Sciences, Milan, Italy.

Journal and publication information

Publication Type: Journal Article

Journal: Therapeutic drug monitoring (Ther Drug Monit), published in UNITED STATES. (Language: eng)

Reference: 2000-Jun; vol 22 (issue 3) : pp 271-6

Dates: Created 2000/09/22; Completed 2000/09/22; Revised 2004/11/17;

PMID: 10850393, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adult Aged Antidepressive Agents, Second-Generation - blood; isolation & purification; pharmacology Chromatography, High Pressure Liquid - methods Depressive Disorder, Major - blood; drug therapy Dose-Response Relationship, Drug Drug Interactions Drug Therapy, Combination Female Fluorometry - methods

Fluoxetine - blood; isolation & purification Fluvoxamine - blood; isolation & purification; pharmacology Humans Linear Models Male Middle Aged Paroxetine - blood; isolation & purification; pharmacology Sensitivity and Specificity Serotonin Uptake Inhibitors - blood; isolation & purification; pharmacology Sertraline - blood; isolation & purification

Associated Chemicals: Antidepressive Agents, Second-Generation (0) ; Serotonin Uptake Inhibitors (0) ; Fluvoxamine (54739-18-3) ; Fluoxetine (54910-89-3) ; Paroxetine (61869-08-7) ; Sertraline (79617-96-2)

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