Research article summary (published 23 May 2001):

The scaffolding protein CASK mediates the interaction between rabphilin3a and beta-neurexins.

Full Abstract

CASK, a member of the membrane-associated guanylate kinase (MAGUK) superfamily, binds to the carboxyl-terminus of beta-neurexins on the intracellular side of the presynaptic membrane. The guanylate kinase-like (GUK) domains of MAGUKs lack kinase activities, but might be important for mediating specific protein-protein interaction. By a yeast two-hybrid approach, we identified an interaction between the GUK domain of CASK and the C2B domain of rabphilin3a, a presynaptic protein involved in synaptic vesicle exocytosis. The interaction was confirmed by in vitro GST pull-down and co-immunoprecipitation assays. It was proposed that presynaptic vesicles might be guided to the vicinity of points of exocytosis defined by beta-neurexins via the interaction between rabphilin3a-CASK-beta-neurexins.

Author information

Author/s: Zhang, Y (Y); Luan, Z (Z); Liu, A (A); Hu, G (G);

Affiliation: Max-Planck Guest Laboratory, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 320, Yue-Yang Road, 200031, Shanghai, PR China.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: FEBS letters (FEBS Lett), published in Netherlands. (Language: eng)

Reference: 2001-May; vol 497 (issue 2-3) : pp 99-102

Dates: Created 2001/05/29; Completed 2001/07/05; Revised 2007/11/15;

PMID: 11377421, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adaptor Proteins, Signal Transducing Animals Blotting, Western COS Cells Calcium-Calmodulin-Dependent Protein Kinases Exocytosis Glutathione Transferase - genetics; metabolism Guanylate Kinase Nerve Tissue Proteins - metabolism Nucleoside-Phosphate Kinase - metabolism; pharmacology

Nucleoside-Phosphate Kinase - metabolism; pharmacology Precipitin Tests Protein Binding - drug effects; physiology Protein Isoforms - metabolism Protein Structure, Tertiary - physiology Recombinant Fusion Proteins - genetics; metabolism Synaptic Vesicles - metabolism Two-Hybrid System Techniques Vesicular Transport Proteins rab GTP-Binding Proteins - metabolism

Associated Chemicals: Adaptor Proteins, Signal Transducing (0) ; Nerve Tissue Proteins (0) ; Protein Isoforms (0) ; Recombinant Fusion Proteins (0) ; Vesicular Transport Proteins (0) ; neurexin II (0) ; rabphilin-3A (0) ; neurexin IIIbeta (156532-79-5) ; neurexin Ibeta (156532-80-8) ; Glutathione Transferase (EC 2.5.1.18) ; Calcium-Calmodulin-Dependent Protein Kinases (EC 2.7.11.17) ; CASK kinases (EC 2.7.4.-) ; Nucleoside-Phosphate Kinase (EC 2.7.4.4) ; Guanylate Kinase (EC 2.7.4.8) ; rab GTP-Binding Proteins (EC 3.6.1.-)

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