Research article summary (published 29 Sep 2001):

Phagocytosis of fibronectin and collagens type I, III, and V by human gingival and periodontal ligament fibroblasts in vitro.

Full Abstract

BACKGROUND: Electron microscopic studies have suggested that the volume density of collagen-containing vacuoles in fibroblasts is higher in the periodontal ligament (PDL) than in the gingiva. Whether this difference reflects intrinsic differences in phagocytic capacity among the cells in these tissues is not known. METHODS: PDL and gingival fibroblasts were isolated from subjects and cultured under identical conditions in the presence of fluorescent beads coated with collagen type I, III, or V or fibronectin. Control beads were coated with bovine serum albumin or an enamel matrix protein mixture that does not constitute part of the extracellular matrix of PDL and gingiva. After various time intervals (1 to 24 hours), the percentage of cells that had internalized beads was assessed by flow cytometry. Since alkaline phosphatase activity has been suggested to play a role in collagen phagocytosis, the activity of this enzyme was determined for all cell populations. RESULTS: The results demonstrated the following order in the percentage of cells internalizing protein-coated beads: fibronectin > collagen type I > III > V. Internalization of collagen type I-coated beads exceeded that of beads coated with bovine serum albumin or enamel matrix proteins by 6 and 3 times, respectively. No differences were observed in collagen phagocytic activity between PDL and gingival fibroblasts, and no relationship could be demonstrated between collagen phagocytosis and alkaline phosphatase activity. CONCLUSIONS: We conclude that differences in collagen phagocytosis between PDL and gingiva, as observed in vivo, are not likely to be explained in terms of intrinsic phagocytic capacities of these cells.

Author information

Author/s: van der Pauw, M T (MT); Van den Bos, T (T); Everts, V (V); Beertsen, W (W);

Affiliation: Department of Periodontology, Academic Center for Dentistry Amsterdam (ACTA), University of Amsterdam, The Netherlands.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of periodontology (J Periodontol), published in United States. (Language: eng)

Reference: 2001-Oct; vol 72 (issue 10) : pp 1340-7

Dates: Created 2001/11/08; Completed 2002/01/16; Revised 2007/11/15;

PMID: 11699475, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adolescent Adult Alkaline Phosphatase - analysis Analysis of Variance Animals Cattle Cells, Cultured Collagen Type I - metabolism Collagen Type III - metabolism Collagen Type V - metabolism Cytochalasin B - pharmacology DNA - analysis

Dental Enamel Proteins - metabolism Fibroblasts - drug effects; metabolism Fibronectins - metabolism Flow Cytometry Gingiva - cytology; drug effects; metabolism Humans Microfilaments - drug effects Microscopy, Electron Periodontal Ligament - cytology; drug effects; metabolism Phagocytosis - drug effects; physiology Serum Albumin, Bovine - metabolism Statistics as Topic

Associated Chemicals: Collagen Type I (0) ; Collagen Type III (0) ; Collagen Type V (0) ; Dental Enamel Proteins (0) ; Fibronectins (0) ; Serum Albumin, Bovine (0) ; Cytochalasin B (14930-96-2) ; DNA (9007-49-2) ; Alkaline Phosphatase (EC 3.1.3.1)

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