Research article summary (published 29 Nov 2001):

Hymenoptera-venom-induced upregulation of the basophil activation marker ecto-nucleotide pyrophosphatase/phosphodiesterase 3 in sensitized individuals.

Full Abstract

BACKGROUND: Bee and wasp venoms are potent allergens capable of inducing severe clinical reactions. To detect immediate-type hypersensitivity to these allergens, a rapid in vitro test was developed that relies on the upregulation of ecto-nucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3) on activated basophils. METHODS: Blood basophils of 13 healthy donors and 22 patients allergic to bee or wasp venom were analyzed for E-NPP3 (CD203c) expression using monoclonal antibody 97A6. Basophils were analyzed by flow cytometry after activation with anti-IgE antibody or allergen. Venom-induced E-NPP3 upregulation on basophils was compared with diagnostic parameters, including skin tests and assessment of specific IgE. In selected samples, the increase in E-NPP3 expression on activated basophils was compared with histamine release and CD63 upregulation. RESULTS: In 20/22 patients sensitized to wasp or bee venom, E-NPP3 expression on basophils was upregulated in response to activation by allergen or anti-IgE. The maximum increase in E-NPP3 expression (above ten times of baseline) was achieved after 15 min of stimulation with 1 microg/ml of allergen or anti-IgE antibody. Sensitized individuals who failed to upregulate E-NPP3 in response to IgE receptor cross-linking also failed to induce histamine release and CD63 upregulation. CONCLUSIONS: Flow cytometric determination of hymenoptera-venom-induced upregulation of E-NPP3 is a novel in vitro test to identify sensitized individuals. Copyright 2002 S. Karger AG, Basel

Author information

Author/s: Platz, I J (IJ); Binder, M (M); Marxer, A (A); Lischka, G (G); Valent, P (P); Bühring, H J (HJ);

Affiliation: University of Tübingen, Department of Internal Medicine II, Division of Hematology and Immunology, Tübingen, Germany.

Journal and publication information

Publication Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't

Journal: International archives of allergy and immunology (Int Arch Allergy Immunol), published in Switzerland. (Language: eng)

Reference: 2001-Dec; vol 126 (issue 4) : pp 335-42

Dates: Created 2002/01/29; Completed 2002/03/08; Revised 2006/11/15;

PMID: 11815741, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

Comments and Corrections

ErratumIn: Int Arch Allergy Immunol 2002 Apr;127(4):293.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adult Aged Allergens - adverse effects; immunology Animals Antigens, CD - metabolism Basophils - immunology; metabolism Bee Venoms - adverse effects; immunology Female Flow Cytometry Histamine Release Humans

Humans Hymenoptera - immunology Hypersensitivity, Immediate - immunology Immunoglobulin E - analysis Male Middle Aged Phosphoric Diester Hydrolases - metabolism Platelet Membrane Glycoproteins - metabolism Pyrophosphatases - metabolism Up-Regulation Wasp Venoms - adverse effects; immunology

Associated Chemicals: Allergens (0) ; Antigens, CD (0) ; Bee Venoms (0) ; ENPP3 protein, human (0) ; Platelet Membrane Glycoproteins (0) ; Wasp Venoms (0) ; lysosomal protein GP53 (0) ; Immunoglobulin E (37341-29-0) ; Phosphoric Diester Hydrolases (EC 3.1.4.-) ; Pyrophosphatases (EC 3.6.1.-)

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