Research article summary (published 30 Jan 2002):

Interaction of synaptic scaffolding molecule and Beta -catenin.

Full Abstract

Synaptic scaffolding molecule (S-SCAM) is a synaptic membrane-associated guanylate kinase with inverted domain organization (MAGI) that interacts with NMDA receptor subunits and neuroligin. In epithelial cells, the non-neuronal isoform of S-SCAM (MAGI-1) is localized at tight or adherens junctions. Recent studies have revealed that the polarized targeting of MAGI-1 to the lateral membrane is mediated by its C-terminal region and that MAGI-1 interacts with beta-catenin in epithelial cells. In this article, we report that S-SCAM interacts with beta-catenin in neurons. beta-Catenin is coimmunoprecipitated with S-SCAM from rat brain. Both S-SCAM and beta-catenin are localized at synapses and are partially colocalized. The C-terminal region of S-SCAM binds to the C-terminal region of beta-catenin. We have tested how the interaction between S-SCAM and beta-catenin plays a role in the synaptic targeting of S-SCAM and beta-catenin. S-SCAM is targeted to synapses via the C-terminal postsynaptic density-95/Dlg-A/ZO-1 (PDZ) domain. beta-Catenin is targeted to synapses with armadillo repeats. The overexpressed C-terminal region of beta-catenin blocks the synaptic targeting of S-SCAM. The overexpressed C-terminal region of S-SCAM is partially targeted to synapses and forms a small number of clusters. In the presence of overexpressed beta-catenin, the C-terminal region of S-SCAM forms more clusters at synapses. These data suggest that the synaptic targeting of S-SCAM is mediated by the interaction with beta-catenin.

Author information

Author/s: Nishimura, Wataru (W); Yao, Ikuko (I); Iida, Junko (J); Tanaka, Noriaki (N); Hata, Yutaka (Y);

Affiliation: Department of Medical Biochemistry, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo 113-8519, Japan.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience (J Neurosci), published in United States. (Language: eng)

Reference: 2002-Feb; vol 22 (issue 3) : pp 757-65

Dates: Created 2002/02/04; Completed 2002/02/27; Revised 2006/11/15;

PMID: 11826105, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article
(including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals COS Cells Carrier Proteins - genetics; metabolism Cells, Cultured Cricetinae Cytoskeletal Proteins - genetics; metabolism Genes, Reporter Green Fluorescent Proteins Guanylate Kinase Hippocampus - cytology; metabolism Luminescent Proteins - genetics Neurons - cytology; metabolism Nucleoside-Phosphate Kinase - metabolism

Nucleoside-Phosphate Kinase - metabolism Precipitin Tests Protein Binding - physiology Protein Structure, Tertiary - genetics; physiology Protein Subunits Rats Receptors, N-Methyl-D-Aspartate - metabolism Recombinant Fusion Proteins - genetics; metabolism Synapses - metabolism Trans-Activators Transfection Two-Hybrid System Techniques beta Catenin

Associated Chemicals: Acvrip1 protein, rat (0) ; Carrier Proteins (0) ; Catnb protein, rat (0) ; Cytoskeletal Proteins (0) ; Luminescent Proteins (0) ; Protein Subunits (0) ; Receptors, N-Methyl-D-Aspartate (0) ; Recombinant Fusion Proteins (0) ; Trans-Activators (0) ; beta Catenin (0) ; Green Fluorescent Proteins (147336-22-9) ; Nucleoside-Phosphate Kinase (EC 2.7.4.4) ; Guanylate Kinase (EC 2.7.4.8)

Related articles

These are the highest related articles currently in the database:

• PICK1 and phosphorylation of the glutamate receptor 2 (GluR2) AMPA receptor subunit regulates GluR2 recycling after NMDA receptor-induced internalization. 10 Dec 2007
• Vascular endothelial growth factor (VEGF) signaling regulates hippocampal neurons by elevation of intracellular calcium and activation of calcium/calmodulin protein kinase II and mammalian target of rapamycin. 15 Dec 2007
• PICK1 uncoupling from mGluR7a causes absence-like seizures. 18 Jul 2008
• The clustering of GABA(A) receptor subtypes at inhibitory synapses is facilitated via the direct binding of receptor alpha 2 subunits to gephyrin. 4 Feb 2008
• Endogenous homer proteins regulate metabotropic glutamate receptor signaling in neurons. 18 Aug 2008
• Erbin controls dendritic morphogenesis by regulating localization of delta-catenin. 7 Jul 2008
• GFP Reconstitution Across Synaptic Partners (GRASP) defines cell contacts and synapses in living nervous systems. 5 Feb 2008
• Regulation of glycogen synthesis by the laforin-malin complex is modulated by the AMP-activated protein kinase pathway. 18 Nov 2007
• Gephyrin selective intrabodies as a new strategy for studying inhibitory receptor clustering. 14 Nov 2007
• Dystonin/Bpag1 is a necessary endoplasmic reticulum/nuclear envelope protein in sensory neurons. Jul 2008

See 100+ related articles.

Related Article Map

Legend: - FREE Full text Article. - Abstract only. - Title only. More help.

See a large map of 100+ related articles.