Research article summary (published 5 Mar 2002):

In vivo regulation of the mandibular organ in the edible crab, Cancer pagurus.

Full Abstract

Considerable evidence indicates that methyl farnesoate (MF) production by the crustacean mandibular organs is negatively regulated by neuropeptides from the sinus gland (SG) in the eyestalk. In the crab Cancer pagurus, two neuropeptides (MO-IH-1 and -2) have been isolated from the SG that inhibit MF synthesis by mandibular organs of female crabs in vitro. To test their activity in vivo, we treated eyestalk-ablated male crabs with SG extracts (SGEs) or MO-IH-1 and -2. SGEs reduced haemolymph levels of MF by 60-80%, while MO-IH-1 and -2 had little effect. Protease treatment of SGEs destroyed the in vivo activity, suggesting that the extract contains an additional peptide responsible for the in vivo activity. When separated by reversed-phase high performance liquid chromatography (HPLC), the in vivo activity eluted in fractions prior to MO-IH-1 and -2. When mandibular organs were removed from animals previously treated in vivo with these active fractions, they had reduced levels of MF synthesis and activity of farnesoic acid O-methyl transferase compared with mandibular organs from animals treated with saline. Together, these results indicate that the regulation of the crustacean mandibular organ is complex and may involve several SG compounds. Some of these compounds (i.e., MO-IH-1 and -2) act directly on the tissue while others affect the mandibular organ indirectly.

Author information

Author/s: Borst, D W (DW); Wainwright, G (G); Rees, H H (HH);

Affiliation: Department of Biological Sciences, Illinois State University, Normal, IL 61790-4120, USA. dwborst(-atsign-)ilstu.edu

Grants: R15 HD 37953-01 (Agency:NICHD NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.

Journal: Proceedings. Biological sciences / The Royal Society (Proc Biol Sci), published in England. (Language: eng)

Reference: 2002-Mar; vol 269 (issue 1490) : pp 483-90

Dates: Created 2002/03/11; Completed 2002/07/11; Revised 2008/11/20;

PMID: 11886640, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article
(including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Brachyura - anatomy & histology; drug effects; physiology Cell Extracts - chemistry; pharmacology Chromatography, High Pressure Liquid Fatty Acids, Unsaturated - analysis; pharmacology; physiology

Hemolymph - drug effects; metabolism Male Neuropeptides - analysis; pharmacology; physiology Organ Specificity Radioimmunoassay

Associated Chemicals: Cell Extracts (0) ; Fatty Acids, Unsaturated (0) ; Neuropeptides (0) ; mandibular organ-inhibiting hormone 1 (0) ; mandibular organ-inhibiting hormone 2 (0) ; methyl farnesoate (10485-70-8)

Related articles

These are the highest related articles currently in the database:

• Molecular characterization and expression of mandibular organ-inhibiting hormone, a recently discovered neuropeptide involved in the regulation of growth and reproduction in the crab Cancer pagurus. 13 Oct 1999
• Ecdysis of decapod crustaceans is associated with a dramatic release of crustacean cardioactive peptide into the haemolymph. 30 Jan 2000
• Melatonin cycle in the fiddler crab Uca pugilator and influence of melatonin on limb regeneration. 29 Sep 1997
• Acute and chronic effects of cadmium on blood homeostasis of an estuarine crab, Chasmagnathus granulata, and the modifying effect of salinity. 30 Mar 2001
• Differential induction of branchial carbonic anhydrase and NA(+)/K(+) ATPase activity in the euryhaline crab, Carcinus maenas, in response to low salinity exposure. 30 May 2002
• Sodium extrusion by the sea-water-acclimated fiddler crab Uca pugilator: comparison with other marine crustacea and marine teleost fish. 30 Jan 1976
• Allatostatin decreases stomatogastric neuromuscular transmission in the crab Cancer borealis. 29 Nov 1997
• Expression and release patterns of neuropeptides during embryonic development and hatching of the green shore crab, Carcinus maenas. 23 Aug 2004
• Effects of eyestalk ablation on carbonic anhydrase activity in the euryhaline blue crab Callinectes sapidus: neuroendocrine control of enzyme expression. 30 Dec 2005

See 100+ related articles.

Related Article Map

Legend: - FREE Full text Article. - Abstract only. - Title only. More help.

See a large map of 100+ related articles.