Effects of laser-induced thermotherapy (LITT) on proliferation and apoptosis of glioma cells in rat brain transplantation tumors.

Full Abstract

BACKGROUND AND OBJECTIVES: Laser-induced thermotherapy (LITT) is an approach to the treatment of brain tumors especially in poorly accessible regions. Its clinical applicability with tumor cell destruction has been shown in several studies. However, no data are known about specific effects on tumors cells due to LITT in the time course of the lesion. STUDY DESIGN/MATERIALS AND METHODS: LITT was performed in adult Lewis rats with implanted glioma cells in the brain using a standard exposure of 3 W for 30 seconds. Before and following LITT, neoplastic lesions were monitored by MRI. Proliferation of implanted cells and gliosis were assessed by several histological techniques and immunohistochemistry. Apoptosis was detected by TUNEL staining. RESULTS: Our experiments show a destruction of neoplastic cells by LITT but surviving tumor cells at the margin of the lesion. Apoptosis was detected following LITT restricted to residual neoplastic cells. Marginal survival of tumor cells lead to a secondary outgrowth into the necrotic lesion adjacent to sprouting capillaries. CONCLUSIONS: LITT is a suitable technique for the treatment of brain neoplasms. However, further investigations are necessary to prevent tumor recurrences after LITT. Copyright 2002 Wiley-Liss, Inc.

Author information

Author/s: Schulze, Paul Christian (PC); Adams, Volker (V); Busert, Christoph (C); Bettag, Martin (M); Kahn, Thomas (T); Schober, Ralf (R);

Affiliation: University of Leipzig, Heart Center, Department of Cardiology, Russenstrasse 19, 04289 Leipzig, Germany.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Lasers in surgery and medicine (Lasers Surg Med), published in United States. (Language: eng)

Reference: 2002-; vol 30 (issue 3) : pp 227-32

Dates: Created 2002/03/13; Completed 2002/10/30; Revised 2006/11/15;

PMID: 11891743, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

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