Research article summary (published 13 Jun 2002):

Limnanthes douglasii lysophosphatidic acid acyltransferases: immunological quantification, acyl selectivity and functional replacement of the Escherichia coli plsC gene.

Full Abstract

Antibodies were raised against the two membrane-bound lysophosphatidic acid acyltransferase (LPAAT) enzymes from Limnanthes douglasii (meadowfoam), LAT1 and LAT2, using the predicted soluble portion of each protein as recombinant protein antigens. The antibodies can distinguish between the two acyltransferase proteins and demonstrate that both migrate in an anomalous fashion on SDS/PAGE gels. The antibodies were used to determine that LAT1 is present in both leaf and developing seeds, whereas LAT2 is only detectable in developing seeds later than 22 daf (days after flowering). Both proteins were found exclusively in microsomal fractions and their amount was determined using the recombinant antigens as quantification standards. LAT1 is present at a level of 27 pg/microg of membrane protein in leaf tissue and

Author information

Author/s: Brown, Adrian P (AP); Carnaby, Simon (S); Brough, Clare (C); Brazier, Melissa (M); Slabas, Antoni R (AR);

Affiliation: Department of Biological Sciences, University of Durham, South Road, Durham DH1 3LE, UK.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: The Biochemical journal (Biochem J), published in England. (Language: eng)

Reference: 2002-Jun; vol 364 (issue Pt 3) : pp 795-805

Dates: Created 2002/06/06; Completed 2002/08/15; Revised 2008/11/20;

PMID: 12049644, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: 18 Feb 2009 00:00:00)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

1-Acylglycerol-3-Phosphate O-Acyltransferase Acyltransferases - analysis; genetics; metabolism Amino Acid Sequence Antibodies Antibody Specificity Blotting, Western Cell Membrane - enzymology Cloning, Molecular Escherichia coli - enzymology; genetics

Escherichia coli - enzymology; genetics Escherichia coli Proteins Immunoassay Isoenzymes - analysis; genetics; metabolism Kinetics Molecular Sequence Data Plant Stems - enzymology Plants - enzymology Recombinant Proteins - metabolism

Associated Chemicals: Antibodies (0) ; Escherichia coli Proteins (0) ; Isoenzymes (0) ; Recombinant Proteins (0) ; Acyltransferases (EC 2.3.-) ; 1-Acylglycerol-3-Phosphate O-Acyltransferase (EC 2.3.1.51) ; plsC protein, E coli (EC 2.3.1.51) ; 2-acylglycerophosphate acyltransferase (EC 2.3.1.52)

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