Research article summary (published 29 Jun 2002):

Topography of the vanilloid receptor in the human bladder: more than just the nerve fibers.

Full Abstract

PURPOSE: We determined the presence and distribution of vanilloid receptor-1 in the human bladder and confirmed or rejected previous findings of other groups that used indirect methods or vanilloid receptor-1 antibodies made by immunizing experimental animals. Also, we tested the reproducibility of results using commercially available antibodies against the N-terminus and C-terminus of the vanilloid receptor. MATERIALS AND METHODS: A total of 11 normal bladder tissue samples were obtained from cystectomy specimens and fresh frozen processed. Specimens were studied by immunohistochemistry and confocal laser microscopy using 3 vanilloid receptor-1 antibodies. Immunohistochemical co-localization studies for neurofilament, neuronal nitric oxide synthase and nerve growth factor were performed. RESULTS: Our results confirm the presence of vanilloid receptor-1 on nonmyelinated and myelinated nerve fibers. There was vanilloid receptor-1 immunoreactivity on smooth muscle cells but different sensitivities for the antibodies. There was immunoreactivity on interstitial cells located in the suburothelium and intermuscular septa of the muscularis. There was co-localization of neuronal nitric oxide synthase with interstitial cells but not with neurofilament. No co-localization was found for nerve growth factor and vanilloid receptor-1. CONCLUSIONS: Vanilloid receptor-1 is located on small unmyelinated and myelinated nerve fibers. In addition, vanilloid receptor-1 is also present on interstitial cells in the suburothelium. There is smooth muscle cell immunoreactivity but a difference in antibodies raised against the C-terminus and N-terminus. These data suggest that the current hypothesis about the mechanism of action of vanilloids is through blocking the afferent reflex arc must be revised and the function of interstitial cells deserves further attention.

Author information

Author/s: Ost, Dieter (D); Roskams, Tania (T); Van Der Aa, Frank (F); De Ridder, Dirk (D);

Affiliation: Department of Urology, University Hospital Gasthuisberg, Leuven, Belgium.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: The Journal of urology (J Urol), published in United States. (Language: eng)

Reference: 2002-Jul; vol 168 (issue 1) : pp 293-7

Dates: Created 2002/06/06; Completed 2002/07/08; Revised 2006/11/15;

PMID: 12050559, status: MEDLINE (last retrieved date: 2/18/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

Comments and Corrections

CommentIn: J Urol. 2003 Feb;169(2):621-2; author reply 622. (PMID: 12544327)

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MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Humans Image Processing, Computer-Assisted Imaging, Three-Dimensional Microscopy, Confocal Microscopy, Fluorescence Nerve Fibers - ultrasonography Nerve Fibers, Myelinated - ultrasonography Nerve Growth Factor - analysis

Nerve Growth Factor - analysis Neurofilament Proteins - analysis Nitric Oxide Synthase - analysis Nitric Oxide Synthase Type I Receptors, Drug - ultrastructure Reproducibility of Results TRPV Cation Channels Urinary Bladder - anatomy & histology; innervation

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: Neurofilament Proteins (0) ; Receptors, Drug (0) ; TRPV Cation Channels (0) ; vanilloid receptor subtype 1 (0) ; Nerve Growth Factor (9061-61-4) ; NOS1 protein, human (EC 1.14.13.39) ; Nitric Oxide Synthase (EC 1.14.13.39) ; Nitric Oxide Synthase Type I (EC 1.14.13.39)

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