Research article summary (published 29 Jun 2002):

Recombinant allergens promote expression of CD203c on basophils in sensitized individuals.

Full Abstract

BACKGROUND: Traditionally, the diagnosis of type I allergies is based on clinical data, skin test results, and laboratory test results with allergen extracts. During the past few years, several attempts have been made to refine diagnostic assays in clinical allergy by introducing recombinant allergens and novel markers of IgE-dependent cell activation. OBJECTIVES: We have identified the ectoenzyme CD203c as a novel basophil antigen that is upregulated on IgE receptor cross-linkage. In this study we applied CD203c and a panel of recombinant allergens to establish a novel basophil test that allows for a reliable quantification of IgE-dependent responses at the effector cell level. METHODS: Patients allergic to birch (Bet v 1, n = 15; Bet v 2, n = 8) and grass (Phl p 1, n = 15; Phl p 2, n = 10; Phl p 5, n = 14) pollen allergens, as well as 10 nonallergic donors, were examined. Basophils were exposed to various concentrations of recombinant allergens for 15 minutes and then examined for expression of CD203c by means of flow cytometry. CD203c upregulation was correlated with the increase in CD63. RESULTS: Exposure to recombinant allergens resulted in a dose-dependent increase in expression of CD203c on peripheral blood basophils in sensitized individuals, whereas no increase was seen in healthy control subjects. The effects of the recombinant allergens on CD203c expression were also time dependent. There was a good correlation between allergen-induced upregulation of CD203c and upregulation of CD63 (R = 0.76). CONCLUSION: Flow cytometric quantitation of CD203c on blood basophils exposed to recombinant allergens is a useful approach to determine the allergic state in sensitized individuals and represents a basis for a sensitive novel allergy test.

Author information

Author/s: Hauswirth, Alexander W (AW); Natter, Susanne (S); Ghannadan, Minoo (M); Majlesi, Yasamin (Y); Schernthaner, Gerit-Holger (GH); Sperr, Wolfgang R (WR); Bühring, Hans-Jörg (HJ); Valenta, Rudolf (R); Valent, Peter (P);

Affiliation: Department of Internal Medicine I, Division of Hematology and Hemostaseology, University of Vienna, Vienna, Austria.

Journal and publication information

Publication Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't

Journal: The Journal of allergy and clinical immunology (J Allergy Clin Immunol), published in United States. (Language: eng)

Reference: 2002-Jul; vol 110 (issue 1) : pp 102-9

Dates: Created 2002/07/11; Completed 2002/09/04; Revised 2006/11/15;

PMID: 12110828, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Allergens - administration & dosage; immunology Antigens, CD - metabolism Basophils - immunology Betula - immunology Female Flow Cytometry - methods Histamine Release Humans Hypersensitivity, Immediate - diagnosis; etiology; immunology

Immunoglobulin E - blood Male Phosphoric Diester Hydrolases - metabolism Plant Proteins - administration & dosage; immunology Platelet Membrane Glycoproteins - metabolism Poaceae - immunology Pyrophosphatases - metabolism Recombinant Proteins - immunology Up-Regulation

Associated Chemicals: Allergens (0) ; Antigens, CD (0) ; ENPP3 protein, human (0) ; Plant Proteins (0) ; Platelet Membrane Glycoproteins (0) ; Recombinant Proteins (0) ; lysosomal protein GP53 (0) ; Immunoglobulin E (37341-29-0) ; Phosphoric Diester Hydrolases (EC 3.1.4.-) ; Pyrophosphatases (EC 3.6.1.-)

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