Research article summary (published 30 Aug 2002):

Simultaneous analysis of nicotine, nicotine metabolites, and tobacco alkaloids in serum or urine by tandem mass spectrometry, with clinically relevant metabolic profiles.

Full Abstract

BACKGROUND: Assessment of nicotine metabolism and disposition has become an integral part of nicotine dependency treatment programs. Serum nicotine concentrations or urine cotinine concentrations can be used to guide nicotine patch dose to achieve biological concentrations adequate to provide the patient with immediate relief from nicotine withdrawal symptoms, an important factor in nicotine withdrawal success. Absence of nicotine metabolites and anabasine can be used to document abstinence from tobacco products, an indicator of treatment success. METHODS: The procedure was designed to quantify nicotine, cotinine, trans-3'-hydroxycotinine, anabasine, and nornicotine in human serum or urine. The technique required simple extraction of the sample with quantification by HPLC-tandem mass spectrometry. RESULTS: The procedure for simultaneous analysis of nicotine, its metabolites, and tobacco alkaloids simultaneously quantified five different analytes. Test limit of quantification, linearity, imprecision, and accuracy were adequate for clinical evaluation of patients undergoing treatment for tobacco dependency. The test readily distinguished individuals who had no exposure to tobacco products from individuals who were either passively exposed or were abstinent past-tobacco users from those who were actively using a tobacco or nicotine product. CONCLUSIONS: Nicotine, cotinine, trans-3'-hydroxycotinine, nornicotine, and anabasine can be simultaneously and accurately quantified in either serum or urine by HPLC-tandem mass spectrometry with imprecision <10% at physiologic concentrations and limits of quantification ranging from 0.5 to 5 micro g/L. Knowledge of serum or urine concentrations of these analytes can be used to guide nicotine replacement therapy or to assess tobacco abstinence in nicotine dependency treatment. These measurements are now an integral part of the clinical treatment and management of patients who wish to overcome tobacco dependence.

Author information

Author/s: Moyer, Thomas P (TP); Charlson, Joel R (JR); Enger, Robert J (RJ); Dale, Lowell C (LC); Ebbert, Jon O (JO); Schroeder, Darrell R (DR); Hurt, Richard D (RD);

Affiliation: Drug Laboratory, Department of Laboratory Medicine & Pathology, Mayo Clinic, 200 1st St. SW, Rochester, MN 55905, USA. moyer.thomas(-atsign-)mayo.edu

Grants: NCTT 6N99C4 (Agency:CIT NIH HHS) ; R01 AA 95005 (Agency:NIAAA NIH HHS) ; R01 CA 90884-01 (Agency:NCI NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.

Journal: Clinical chemistry (Clin Chem), published in United States. (Language: eng)

Reference: 2002-Sep; vol 48 (issue 9) : pp 1460-71

Dates: Created 2002/08/26; Completed 2002/09/13; Revised 2007/11/14;

PMID: 12194923, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adult Alkaloids - analysis; blood; urine Anabasine - blood; urine Cotinine - analogs & derivatives; blood; urine Female Humans Male

Male Mass Spectrometry Middle Aged Nicotine - analogs & derivatives; analysis; blood; urine Reference Values Substance Abuse Detection - methods Tobacco

Associated Chemicals: Alkaloids (0) ; hydroxycotinine (27323-64-4) ; Cotinine (486-56-6) ; Anabasine (494-52-0) ; Nicotine (54-11-5) ; nornicotine (5746-86-1)

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