Research article summary (published 25 Sep 2002):

Enhancement of bone repair with a helper-dependent adenoviral transfer of bone morphogenetic protein-2.

Full Abstract

Regional gene therapy, which involves the delivery of growth factors to a specific anatomic site, has the potential to enhance bone formation in clinical application. Helper-dependent adenoviral vectors, which have deleted all of the viral coding regions, have been shown to be safe and highly efficient with long-lasting transgene expression. In this study, we constructed a helper-dependent adenoviral vector producing bone morphogenetic protein-2 (AdHDBMP-2). The AdHDBMP-2 increased the alkaline phosphatase activity of W-20-17 cells in vitro. In addition, when AdHDBMP-2 infected rat bone marrow cells were implanted into the hindlimbs of SCID mice, orthotopic bone formation was shown at 2 weeks. To our knowledge, this is the first study to demonstrate bone formation with the helper-dependent adenoviral vector with the BMP-2 expression cassette. This type of gene therapy vector could prove to be highly useful for bone augmentation in patients with bone loss associated with trauma, revision total joint arthroplasty, or cancer.

Author information

Author/s: Abe, Nobuhiro (N); Lee, Yu-Po (YP); Sato, Makoto (M); Zhang, Xuguang (X); Wu, Jing (J); Mitani, Kohnosuke (K); Lieberman, Jay R (JR);

Affiliation: Department of Orthopaedic Surgery, UCLA School of Medicine, 2-619 MacDonald Research Laboratories, 675 Charles E. Young Dr. South, Los Angeles, CA 90095, USA.

Grants: AR 46789 (Agency:NIAMS NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, U.S. Gov't, P.H.S.

Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun), published in United States. (Language: eng)

Reference: 2002-Sep; vol 297 (issue 3) : pp 523-7

Dates: Created 2002/09/24; Completed 2002/11/07; Revised 2008/11/21;

PMID: 12270126, status: MEDLINE (last retrieved date: 2/18/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Adenoviridae Alkaline Phosphatase - genetics Animals Base Sequence Bone Development - genetics; physiology Bone Marrow Cells - cytology Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins - biosynthesis; genetics Cell Line Cells, Cultured Chondrocytes - cytology; physiology DNA Primers Gene Therapy - methods

Gene Therapy - methods Genetic Vectors Mice Mice, SCID Mice, Transgenic Osteoblasts - cytology; physiology Osteogenesis - physiology Polymerase Chain Reaction Rats Rats, Inbred Lew Recombinant Proteins - biosynthesis Transfection Transforming Growth Factor beta

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: Bmp2 protein, mouse (0) ; Bmp2 protein, rat (0) ; Bone Morphogenetic Protein 2 (0) ; Bone Morphogenetic Proteins (0) ; DNA Primers (0) ; Recombinant Proteins (0) ; Transforming Growth Factor beta (0) ; Alkaline Phosphatase (EC 3.1.3.1)

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