Research article summary (published 4 Nov 2002):

Production in large quantities of highly purified hydroxytyrosol from liquid-solid waste of two-phase olive oil processing or "Alperujo".

Full Abstract

The effect of hydrothermal treatment of two-phase olive waste (alperujo) on the solubilization of hydroxytyrosol was studied. Different conditions of saturated steam were assayed. A high amount of hydroxytyrosol was solubilized and increased with increasing steaming temperature and time, reaching 1.4-1.7 g/100 g of dry alperujo. The effect of acidic (H(2)SO(4)) and basic (NaOH) catalysts was also evaluated. Acid-catalyzed treatment was more effective at milder conditions, whereas the alkali-catalyzed conditions were not very suitable. In the present study, the extracted hydroxytyrosol was purified by means of a new, simple, and inexpensive chromatographic system, under international patent application (PCT/ES02/00058). From 1000 kg of alperujo, with 70% humidity, can be obtained approximately 4.5-5 kg of hydroxytyrosol. After a purification process, at least 3 kg of hydroxytyrosol, at 90-95% purity, would be obtained. The purified compound was identified by HPLC/UV and (1)H and (13)C NMR analyses, and its antioxidant activity was tested on refined olive oil without antioxidants by Rancimat method. The oxidative stability of refined olive oil was increased by a factor of 1.71 in the presence of 100 ppm of hydroxytyrosol.

Author information

Author/s: Fernández-Bolaños, Juan (J); Rodríguez, Guillermo (G); Rodríguez, Rocio (R); Heredia, Antonia (A); Guillén, Rafael (R); Jiménez, Ana (A);

Affiliation: Food Biotechnology Departament, Instituto de la Grasa (CSIC), Avenida Padre García Tejero 4, Apartado 1078, 41012 Sevilla, Spain. jfbg(-atsign-)cica.es

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of agricultural and food chemistry (J Agric Food Chem), published in United States. (Language: eng)

Reference: 2002-Nov; vol 50 (issue 23) : pp 6804-11

Dates: Created 2002/10/30; Completed 2002/12/26; Revised 2008/11/21;

PMID: 12405779, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Chromatography, High Pressure Liquid Drug Stability Food Handling Hot Temperature Hydrogen-Ion Concentration Industrial Waste

Magnetic Resonance Spectroscopy Oxidation-Reduction Phenylethyl Alcohol - analogs & derivatives; chemistry; isolation & purification Plant Oils - chemistry Solubility Steam

Associated Chemicals: Industrial Waste (0) ; Plant Oils (0) ; Steam (0) ; 3,4-dihydroxyphenylethanol (10597-60-1) ; Phenylethyl Alcohol (60-12-8) ; olive oil (8001-25-0)

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