Research article summary (published 9 Oct 2002):

Purification of (+)-dihydromyricetin from leaves extract of Ampelopsis grossedentata using high-speed countercurrent chromatograph with scale-up triple columns.

Full Abstract

Purification of (+)-dihydromyricetin from an extract (16 g) of leaves of Ampelopsis grossedentata was performed using a preparative triple-column countercurrent chromatograph. With a solvent system composed of n-hexane-ethyl acetate-methanol-water (1:3:2:4, v/v) 11.3 g of (+)-dihydromyricetin was obtained at a high purity of over 99% by HPLC at 254 nm in 9 h.

Author information

Author/s: Du, Qizhen (Q); Cai, Weijian (W); Xia, Ming (M); Ito, Yoichiro (Y);

Affiliation: Institute of Food and Biological Engineering, Hangzhou University of Commerce, China.

Journal and publication information

Publication Type: Journal Article

Journal: Journal of chromatography. A (J Chromatogr A), published in Netherlands. (Language: eng)

Reference: 2002-Oct; vol 973 (issue 1-2) : pp 217-20

Dates: Created 2002/11/19; Completed 2003/04/11; Revised 2009/01/15;

PMID: 12437181, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Ampelopsis - chemistry Chromatography, High Pressure Liquid Countercurrent Distribution - instrumentation; methods Flavonoids - isolation & purification Flavonols

Flavonols Magnetic Resonance Spectroscopy Mass Spectrometry Plant Extracts - chemistry Plant Leaves - chemistry

Associated Chemicals: Flavonoids (0) ; Flavonols (0) ; Plant Extracts (0) ; dihydromyricetin (0)

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