Research article summary (published 30 Jan 1999):

[The modulation of collagen synthesis and expressions of type I and III collagen mRNAs in aorta smooth muscle cell by PDGF and heparin]

Full Abstract

OBJECTIVE: The effect of platelet derived growth factor (PDGF) and heparin on the modulation of human aorta smooth muscle cell(hASMC) proliferation, collagen synthesis, expressions of type I and III collagen mRNAs as well as transforming growth factor-beta (TGF-beta) mRNA were investigated. METHODS: 3H-TdR and 3H-proline incorporation and Northern blot analysis were done. 3H-TdR and 3H-proline incorporation was statistically analyzed with t test among different experimental groups. RESULTS: In comparison to the control group, PDGF possessed the ability in promoting markedly the DNA synthesis (3H-TdR incorporation: PDGF group vs control, P < 0.01), synthesis and secretion of collagen protein (3H-proline incorporation of SMC and medium: PDGF group vs control, P < 0.01), expressions of type I and type III collagen mRNAs, and transforming growth factor mRNA in hASMC. Whereas heparin significantly decreased the DNA synthesis (heparin group vs control, P < 0.01), synthesis and secretion of collagen protein (heparin group vs control, P < 0.01) of hASMC in vitro. Heparin also inhibited the promoting effect of PDGF on DNA synthesis, the synthesis and secretion of collagen protein, and reduced the up-regulation of expressions of type I and type III collagen mRNAs as well as TGF-beta mRNA that stimulated by PDGF. CONCLUSIONS: PDGF could promote the collagen synthesis through upregulating type I and type III collagen mRNA expressions while heparin just inhibiting SMC DNA and collagen synthesis effected in the way of anti-atherosclerosis.

Author information

Author/s: Yang, F (F); Zhao, P (P); She, M (M); Ye, G (G); Han, X (X);

Affiliation: Cardiovascular Institute, Fu Wai Hospital, CAMS and PUMC, Beijing 100037.

Journal and publication information

Publication Type: English Abstract; Journal Article; Research Support, Non-U.S. Gov't

Journal: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae (Zhongguo Yi Xue Ke Xue Yuan Xue Bao), published in China. (Language: chi)

Reference: 1999-Feb; vol 21 (issue 1) : pp 13-8

Dates: Created 2003/02/06; Completed 2003/08/29; Revised 2006/11/15;

PMID: 12569634, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Anticoagulants - pharmacology Aorta, Thoracic - cytology; metabolism Cells, Cultured Collagen Type I - biosynthesis; genetics Collagen Type III - biosynthesis; genetics DNA - biosynthesis Fetus

Heparin - pharmacology Humans Muscle, Smooth, Vascular - cytology; metabolism Platelet-Derived Growth Factor - pharmacology Procollagen - genetics RNA, Messenger - genetics Transforming Growth Factor beta - biosynthesis; genetics

Associated Chemicals: Anticoagulants (0) ; Collagen Type I (0) ; Collagen Type III (0) ; Platelet-Derived Growth Factor (0) ; Procollagen (0) ; RNA, Messenger (0) ; Transforming Growth Factor beta (0) ; Heparin (9005-49-6) ; DNA (9007-49-2)

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