Research article summary (published 29 Nov 2000):

[Effect of amniotic membrane on expressions of TGF-beta 1, collagens I, III and fibronectin in rabbit corneal healing after photorefractive keratectomy]

Full Abstract

OBJECTIVE: To observed the effect of amniotic membrane transplantation (AMT) on expressions of transforming growth factor-beta 1(TGF-beta 1), collagens I, III and fibronectin (FN) in rabbit corneal healing after photorefractive keratectomy (PRK), and investigate the anti-scarring formation mechanism of AMT. METHODS: Ten rabbits underwent bilateral PRK to correct 8 diopters of myopia. One eye was randomly transplanted with preserved human amniotic membrane, and the other eye served as the control. The expressions of TGF-beta 1, collagens I, III and FN were studied by immunohistochemistry at 4 weeks after operation. RESULTS: The expression of TGF-beta 1 in corneal epithelium and keratocytes and collegans in anterior stroma was significantly less in AMT group than in the control group. The expressions of collagens III and FN in anterior stroma of ablation area were also significantly less in AMT group than in the control group. The staining of collagen I had no significant difference between the AMT group and the control group. CONCLUSION: The expressions of TGF-beta 1, collagens III and FN can be suppressed by amniotic membrane after PRK. These data suggest that part of the anti-scarring effect of AMT may be mediated through the suppression of TGF-beta 1, collagens III and FN expression.

Author information

Author/s: Zhong, Y (Y); Zhai, Z (Z); Zhou, Y (Y); Ye, W (W); Wang, K (K);

Affiliation: Ophthalmic Center, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.

Journal and publication information

Publication Type: English Abstract; Journal Article; Research Support, Non-U.S. Gov't

Journal: Yan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu (Yan Ke Xue Bao), published in China. (Language: chi)

Reference: 2000-Dec; vol 16 (issue 4) : pp 239-42, 258

Dates: Created 2003/02/12; Completed 2004/07/23; Revised 2008/11/21;

PMID: 12579778, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Amnion - transplantation Animals Collagen Type I - biosynthesis; genetics Collagen Type III - biosynthesis; genetics Cornea - metabolism Female Fibronectins - biosynthesis; genetics Humans Lasers, Excimer

Lasers, Excimer Male Myopia - metabolism; surgery Photorefractive Keratectomy Rabbits Transforming Growth Factor beta - biosynthesis; genetics Transforming Growth Factor beta1 Transplantation, Heterologous Wound Healing

Associated Chemicals: Collagen Type I (0) ; Collagen Type III (0) ; Fibronectins (0) ; TGFB1 protein, human (0) ; Transforming Growth Factor beta (0) ; Transforming Growth Factor beta1 (0)

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