Research article summary (published 30 Jan 2003):

Contrasting responses of human gingival and periodontal ligament fibroblasts to bacterial cell-surface components through the CD14/Toll-like receptor system.

Full Abstract

We compared human periodontal ligament fibroblasts with human gingival fibroblasts isolated from the same donor to examine interleukin-8 (IL-8) responses of the cells to Salmonella lipopolysaccharide, a water-soluble peptidoglycan from Staphylococcus epidermidis and the synthetic muramyldipeptide, with special reference to the possible involvement of the CD14/Toll-like receptor (TLR) system of the cells in the responses. Human gingival fibroblasts expressed CD14 on their surfaces and strongly expressed CD14 mRNA, while human periodontal ligament fibroblasts showed considerably lower levels of expression in both respects. Both cells expressed mRNA of TLR-related molecules, i.e. TLR2, TLR4, MD-2 and MyD88, although human periodontal ligament fibroblasts expressed TLR2 more strongly than human gingival fibroblasts. Human gingival fibroblasts exhibited a stronger IL-8 response than human periodontal ligament fibroblasts to lipopolysaccharide, while human periodontal ligament fibroblasts exhibited a response comparable to, or slightly stronger than, that of human gingival fibroblasts to S. epidermidis peptidoglycan and muramyldipeptide. The IL-8 responses of both cells to lipopolysaccharide and S. epidermidis peptidoglycan were completely inhibited by antihuman CD14 monoclonal antibody (MAb). The responses of both cells to lipopolysaccaride were significantly inhibited by antihuman TLR4 MAb, while those to S. epidermidis peptidoglycan were inhibited by antihuman TLR2 MAb. In contrast, muramyldipeptide activated both types of cells in a TLR2- and TLR4-independent manner, although the activities of muramyldipeptide on human gingival fibroblasts, but not human periodontal ligament fibroblasts, were significantly inhibited by anti-CD14 MAb.

Author information

Author/s: Hatakeyama, J (J); Tamai, R (R); Sugiyama, A (A); Akashi, S (S); Sugawara, S (S); Takada, H (H);

Affiliation: Department of Microbiology and Immunology,Tohoku University School of Dentistry, Sendai, Japan.

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't

Journal: Oral microbiology and immunology (Oral Microbiol Immunol), published in Denmark. (Language: eng)

Reference: 2003-Feb; vol 18 (issue 1) : pp 14-23

Dates: Created 2003/02/17; Completed 2003/05/21; Revised 2006/11/15;

PMID: 12588454, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Acetylmuramyl-Alanyl-Isoglutamine - immunology Analysis of Variance Antigens, CD14 - physiology Bacterial Proteins - immunology Drosophila Proteins Fibroblasts - immunology; metabolism; microbiology Flow Cytometry Gingiva - cytology; immunology; metabolism; microbiology Gram-Negative Bacteria - immunology Gram-Positive Bacteria - immunology Humans

Interleukin-8 - biosynthesis Lipopolysaccharides - immunology Membrane Glycoproteins - physiology Peptidoglycan - immunology Periodontal Ligament - cytology; immunology; metabolism; microbiology Receptors, Cell Surface - physiology Reverse Transcriptase Polymerase Chain Reaction Statistics, Nonparametric Toll-Like Receptor 2 Toll-Like Receptor 4 Toll-Like Receptors

Associated Chemicals: Antigens, CD14 (0) ; Bacterial Proteins (0) ; Drosophila Proteins (0) ; Interleukin-8 (0) ; Lipopolysaccharides (0) ; Membrane Glycoproteins (0) ; Peptidoglycan (0) ; Receptors, Cell Surface (0) ; TLR2 protein, human (0) ; TLR4 protein, human (0) ; Toll-Like Receptor 2 (0) ; Toll-Like Receptor 4 (0) ; Toll-Like Receptors (0) ; Acetylmuramyl-Alanyl-Isoglutamine (53678-77-6)

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