Research article summary (published 30 Dec 2001):

Transfection of articular chondrocytes with rhBMP7 gene and its expression.

Full Abstract

In order to investigate the possibility of expression of exogenous gene in transduced articular chondrocytes, plasmid pcDNA3-rhBMP7 was delivered to cultured chondrocytes. Through immunohistochemical staining and RT-PCR assay, the expression of rhBMP7 gene was detected. And the bioactivity of transgene expression product was detected through MTT assay as well. It was confirmed that exogenous gene could be expressed efficiently in transduced chondrocytes and the transgene expression product had obvious bioactivity. The present study provided a theoretical basis for gene therapy on the problems of articular cartilage.

Author information

Author/s: Duan, Deyu (D); Du, Jingyuan (J); Liu, Yong (Y); Guo, Xiaodong (X); Wang, Hong (H);

Affiliation: Department of Orthopedics, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.

Journal and publication information

Publication Type: Journal Article

Journal: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban (J Huazhong Univ Sci Technolog Med Sci), published in China. (Language: eng)

Reference: 2002-; vol 22 (issue 1) : pp 42-3

Dates: Created 2003/03/27; Completed 2004/08/09; Revised 2008/11/21;

PMID: 12658780, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Bone Morphogenetic Protein 7 Bone Morphogenetic Proteins - biosynthesis; genetics Cartilage, Articular - metabolism Chondrocytes - metabolism

Gene Expression Rabbits Recombinant Proteins - biosynthesis; genetics Transfection Transforming Growth Factor beta - biosynthesis; genetics; metabolism

Associated Chemicals: Bone Morphogenetic Protein 7 (0) ; Bone Morphogenetic Proteins (0) ; Recombinant Proteins (0) ; Transforming Growth Factor beta (0)

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