The OmpL porin does not modulate redox potential in the periplasmic space of Escherichia coli.

Full Abstract

The Escherichia coli DsbA protein is the major oxidative catalyst in the periplasm. Dartigalongue et al. (EMBO J., 19, 5980-5988, 2000) reported that null mutations in the ompL gene of E.coli fully suppress all phenotypes associated with dsbA mutants, i.e. sensitivity to the reducing agent dithiothreitol (DTT) and the antibiotic benzylpenicillin, lack of motility, reduced alkaline phosphatase activity and mucoidy. They showed that OmpL is a porin and hypothesized that ompL null mutations exert their suppressive effect by preventing efflux of a putative oxidizing-reducing compound into the medium. We have repeated these experiments using two different ompL null alleles in at least three different E.coli K-12 genetic backgrounds and have failed to reproduce any of the ompL suppressive effects noted above. Also, we show that, contrary to earlier results, ompL null mutations alone do not result in partial DTT sensitivity or partial motility, nor do they appreciably affect bacterial growth rates or block propagation of the male-specific bacteriophage M13. Thus, our findings clearly demonstrate that ompL plays no perceptible role in modulating redox potential in the periplasm of E.coli.

Author information

Author/s: Sardesai, Abhijit A (AA); Genevaux, Pierre (P); Schwager, Françoise (F); Ang, Debbie (D); Georgopoulos, Costa (C);

Affiliation: Département de Biochimie Médicale, Centre Médical Universitaire, Université de Genève, 1 rue Michel-Servet, CH-1211 Genève 4, Switzerland.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: The EMBO journal (EMBO J), published in England. (Language: eng)

Reference: 2003-Apr; vol 22 (issue 7) : pp 1461-6

Dates: Created 2003/03/27; Completed 2003/05/14; Revised 2008/11/21;

PMID: 12660153, status: MEDLINE (last retrieved date: 2/18/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Alkaline Phosphatase - metabolism
Bacterial Proteins
Base Sequence
DNA Primers
Dithiothreitol - pharmacology
Escherichia coli - drug effects; enzymology; metabolism
Escherichia coli Proteins

Escherichia coli Proteins
Genes, Bacterial
Mutation
Oxidation-Reduction
Periplasm - drug effects; enzymology; metabolism
Porins - genetics; physiology
Protein Disulfide-Isomerases - genetics

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: Bacterial Proteins (0) ; DNA Primers (0) ; Escherichia coli Proteins (0) ; OmpL protein, E coli (0) ; OmpL protein, bacteria (0) ; Porins (0) ; Dithiothreitol (3483-12-3) ; Alkaline Phosphatase (EC 3.1.3.1) ; Protein Disulfide-Isomerases (EC 5.3.4.1)

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