Research article summary (published 30 Jul 2003):

Effect of middle cerebral artery occlusion on mRNA expression for the sodium-coupled vitamin C transporter SVCT2 in rat brain.

Full Abstract

The sodium-vitamin C co-transporter SVCT2 is primarily responsible for the accumulation of the important antioxidant ascorbate into brain cells. In vitro studies have demonstrated strong expression of this transporter in cultured astrocytes, whereas in situ hybridization analysis has so far detected SVCT2 only in neurons. In the present study, we examined the response of SVCT2 mRNA expression in the brain to focal ischemia induced for 2 h by unilateral middle cerebral artery occlusion. The mRNA expression patterns of SVCT2 and the glutamate-activated immediate early gene Arc were investigated at 2 and 22 h after ischemia. SVCT2 and Arc mRNA expression was lost in the ischemic core at both time points. In areas outside the core, Arc was strongly up-regulated, primarily at 2 h, whereas SVCT2 showed an increase at 2 and 22 h. SVCT2 expression was increased in neurons as well as in astrocytes, providing the first evidence for SVCT2 expression in astrocytes in situ. These findings underscore the importance of ascorbate as a neuroprotective agent and may have implications for therapeutic strategies. In addition, the increase of SVCT2 in astrocytes after ischemia suggests that cultured astrocytes are exposed to chronic oxidative stress.

Author information

Author/s: Berger, Urs V (UV); Lu, Xi-Chun M (XC); Liu, Weilin (W); Tang, Zhaocheng (Z); Slusher, Barbara S (BS); Hediger, Matthias A (MA);

Affiliation: Membrane Biology Program and Renal Division, Brigham and Women's Hospital, Boston, Massachusetts, USA.

Grants: 32001 (Agency:PHS HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.

Journal: Journal of neurochemistry (J Neurochem), published in England. (Language: eng)

Reference: 2003-Aug; vol 86 (issue 4) : pp 896-906

Dates: Created 2003/07/30; Completed 2003/10/01; Revised 2007/11/14;

PMID: 12887688, status: MEDLINE (last retrieved date: 2/18/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article
(including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Animals Astrocytes - metabolism; pathology Brain - blood supply; metabolism; pathology Cytoskeletal Proteins Disease Models, Animal Disease Progression Immediate-Early Proteins - genetics; metabolism In Situ Hybridization Infarction, Middle Cerebral Artery - metabolism; pathology

Infarction, Middle Cerebral Artery - metabolism; pathology Male Nerve Tissue Proteins Neurons - metabolism; pathology Organic Anion Transporters, Sodium-Dependent - genetics RNA, Messenger - metabolism Rats Rats, Sprague-Dawley Symporters - genetics

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: Cytoskeletal Proteins (0) ; Immediate-Early Proteins (0) ; Nerve Tissue Proteins (0) ; Organic Anion Transporters, Sodium-Dependent (0) ; RNA, Messenger (0) ; Symporters (0) ; activity regulated cytoskeletal-associated protein (0) ; sodium-dependent vitamin C transporter 2 (0)

Related articles

These are the most related articles currently in our database:

• Ischemic preconditioning reveals that GLT1/EAAT2 glutamate transporter is a novel PPARgamma target gene involved in neuroprotection. 8 Jan 2007
• Expressions of neuropilin-1, neuropilin-2 and semaphorin 3A mRNA in the rat brain after middle cerebral artery occlusion. 26 Sep 2001
• Dynamic expression of glial cell line-derived neurotrophic factor after cerebral ischemia. 25 Apr 2000
• Monocyte chemoattractant protein-1 expressed in neurons and astrocytes during focal ischemia in mice. 30 May 2001
• Vitamin C uptake and recycling among normal and tumor cells from the central nervous system. 30 Dec 2004
• Pretreatment with melatonin exerts anti-inflammatory effects against ischemia/reperfusion injury in a rat middle cerebral artery occlusion stroke model. 30 Aug 2004
• Fractalkine cleavage from neuronal membranes represents an acute event in the inflammatory response to excitotoxic brain damage. 30 Jul 2000
• Brain-derived neurotrophic factor reduces cortical cell death by ischemia after middle cerebral artery occlusion in the rat. 27 Feb 2001
• Stroke induces widespread changes of gene expression for glial cell line-derived neurotrophic factor family receptors in the adult rat brain. 30 Dec 2000

See 100+ related articles.

Related Article Map

Legend: - FREE Full text Article. - Abstract only. - Title only. More help.

See a larger map of 100+ related articles.