Research article summary (published 30 Aug 2003):

Antisense oligonucleotide against 47-kDa heat shock protein (Hsp47) inhibits wound-induced enhancement of collagen production.

Full Abstract

It is well known that excessive collagen synthesis during the wound-healing process causes scar formation. Our recent in-vivo study indicates that antisense treatment against 47-kDa heat shock protein (Hsp47), a collagen-specific molecular chaperone, relieves scar formation following skin wounds in rats [Wang et al., Plast. Reconstr. Surg., in press]. In order to understand the mechanism of this phenomenon, we examined the effects of antisense treatment on the expression of mRNAs and proteins of Hsp47 and collagens in fibroblasts derived from wounded rat tongues. Hsp47 and procollagen alpha1(I) and alpha1(III) mRNAs were consistently increased after wounding and were maximal at day 5 post-injury. Treatment with antisense oligonucleotide against Hsp47 efficiently blocked the production of procollagen alpha2(I) and alpha1(III) proteins, but had little effect on their mRNA levels. Therefore, we conclude that antisense oligonucleotide against Hsp47 inhibits the production of procollagen type I and III proteins in fibroblasts derived from wounded tongues, overcoming the increase in their mRNAs.

Author information

Author/s: Ohba, S (S); Wang, Z L (ZL); Baba, T T (TT); Nemoto, T K (TK); Inokuchi, T (T);

Affiliation: Division of Oral and Maxillofacial Surgical Reconstruction and Functional Restoration, Department of Developmental and Reconstructive Medicine, Graduate School of Biomedical Sciences, Nagasaki University, Sakamoto, Japan. d499004b(-atsign-)stcc.nagasaki-u.ac.jp

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Archives of oral biology (Arch Oral Biol), published in England. (Language: eng)

Reference: 2003-Sep; vol 48 (issue 9) : pp 627-33

Dates: Created 2003/07/30; Completed 2003/10/08; Revised 2006/11/15;

PMID: 12887997, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: 18 Feb 2009 00:00:00)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Blotting, Western Cells, Cultured Collagen Type I - biosynthesis; genetics Collagen Type III - biosynthesis; genetics Fibroblasts - metabolism Gene Expression Regulation HSP47 Heat-Shock Proteins Heat-Shock Proteins - antagonists & inhibitors; biosynthesis; genetics

Heat-Shock Proteins - antagonists & inhibitors; biosynthesis; genetics Oligonucleotides, Antisense - pharmacology Procollagen - biosynthesis RNA, Messenger - genetics Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction Tongue - injuries; metabolism Wound Healing - physiology

Associated Chemicals: Collagen Type I (0) ; Collagen Type III (0) ; HSP47 Heat-Shock Proteins (0) ; Heat-Shock Proteins (0) ; Oligonucleotides, Antisense (0) ; Procollagen (0) ; RNA, Messenger (0) ; Serpinh1 protein, rat (0)

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