Research article summary (published 29 Jun 2003):

[Therapeutic effects of octreotide on hepatofibrosis-induced with carbon tetrachloride in rats]

Full Abstract

OBJECTIVES: To investigate the therapeutic effects and mechanism of octreotide on experimental hepatic fibrosis in rats. METHODS: Hepatofibrotic rats models were established with carbon tetrachloride. All the experimental rats were divided into four groups: normal control group, pre-and post-treatment model group, and octreotide-treated group in which the rats were injected subcutaneously with octreotide at the dose of 50ng/100g, twice daily, for thirty days. Serum levels of hyaluronic acid (HA), laminin (LN) and pro-collagen type III peptide (PCIII) were detected by radioimmunoassay. Hepatic fibrosis scoring grade was assessed through Van-Gieson staining and observed under light microscope. Protein expression levels of alpha-smooth muscle actin (alpha-SMA) and transforming growth factor beta1 (TGFbeta1) were determined with immunohistochemical staining method. Messenger RNA (mRNA) levels of collagen type I and PCIII were detected by reverse transcription polymerase chain reaction. RESULTS: Serum levels of HA (ng/L), LN (microg/L) and PCIII (ng/L) in pre- and post-treatment model groups were higher than those in normal control group (121.8+/-9.5 and 110.3+/-13.4 vs. 33.1+/-3.7, 85.7+/-12.1 and 78.2+/-7.9 vs. 37.1+/-6.3, 35.9+/-3.5 and 33.7+/-2.6 vs. 15.6+/-2.8, respectively, t > or = 9.41, P<0.05), and there was no significant difference between the two model groups. Concentrations of HA (55.8ng/L+/-7.2ng/L), LN (43.1microg/L+/-3.4microg/L) and PCIII (27.8ng/L+/-3.4ng/L) decreased significantly in octreotide-treated group, compared with those in model groups (t >or=2.76, P<0.05). With histological analysis, fibrotic scoring grade in octreotide-treated group was obviously ameliorated, compared with that in model groups (chi2 > or = 3.97, P<0.05). Imaging analysis revealed that alpha-SMA and TGFbeta1 immunohistological staining areas were markedly shrinked in octreotide-treated group (t > or = 2.47, P < 0.05). In two model groups, PCIII and type I mRNA levels significantly up-regulated as compared with those in normal group (t > or = 9.27, P<0.001), and they were inhibited by octreotide markedly (t > or = 2.47, P<0.05). CONCLUSIONS: Octreotide can inhibit hepatic stellate cells transforming into myofibroblasts, down-regulate TGFbeta1, collagen type I and PCIII transcriptions, so that it has therapeutic effects on experimental hepatic fibrosis.

Author information

Author/s: Wang, Zhi-rong (ZR); Li, Ding-guo (DG); Chen, Xi-mei (XM); Huang, Xin (X); Wei, Hong-shan (HS); Wang, Yu-qin (YQ); Xu, Qin-fang (QF); Lu, Han-ming (HM);

Affiliation: Department of Gastroenterology, Tongji Hospital, Tongji University, Shanghai 200065, China.

Journal and publication information

Publication Type: English Abstract; Journal Article

Journal: Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (Zhonghua Gan Zang Bing Za Zhi), published in China. (Language: chi)

Reference: 2003-Jul; vol 11 (issue 7) : pp 408-11

Dates: Created 2003/07/31; Completed 2004/01/06; Revised 2006/11/15;

PMID: 12890342, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Actins - analysis Animals Carbon Tetrachloride - toxicity Collagen Type I - genetics Collagen Type III - genetics Hyaluronic Acid - blood Laminin - blood Liver - pathology

Liver Cirrhosis, Experimental - drug therapy; metabolism; pathology Male Octreotide - therapeutic use RNA, Messenger - analysis Rats Rats, Sprague-Dawley Transforming Growth Factor beta - analysis Transforming Growth Factor beta1

Associated Chemicals: Actins (0) ; Collagen Type I (0) ; Collagen Type III (0) ; Laminin (0) ; RNA, Messenger (0) ; Tgfb1 protein, rat (0) ; Transforming Growth Factor beta (0) ; Transforming Growth Factor beta1 (0) ; Carbon Tetrachloride (56-23-5) ; Octreotide (83150-76-9) ; Hyaluronic Acid (9004-61-9)

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