Production of intergeneric somatic hybrids between round kumquat (Fortunella japonica Swingle) and 'Morita navel' orange (Citrus sinensis Osbeck).

Full Abstract

Intergeneric somatic hybrids between embryogenic callus-derived protoplasts of round kumquat (Fortunella japonica Swingle) and 'Morita navel' orange (Citrus sinensis Osbeck) were produced by electrofusion. Among the eight different fusion strains obtained, six showed normal morphology, whereas the remaining two showed malformation. All the regenerated plants were intermediate in leaf morphology and had thick and round leaves, which are typical characteristics of polyploids. Ploidy analyses by flow cytometry and chromosome counting in root-tip cells revealed that these plants are amphidiploid (2n=4x=36). Hybridity of the fusion products was confirmed by random amplified polymorphic DNA and cleaved amplified polymorphic sequence (CAPS) analyses. Furthermore, analyses of chloroplast (cp) and mitochondrial (mt) DNA by CAPS showed that these somatic hybrids contained cp- and mt-DNA of round kumquat without recombination in the regions analyzed.

Author information

Author/s: Takami, K (K); Matsumara, A (A); Yahata, M (M); Imayama, T (T); Kunitake, H (H); Komatsu, H (H);

Affiliation: Faculty of Agriculture, Miyazaki University, Gakuenkibanadai, 889-2192, Miyazaki, Japan.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Plant cell reports (Plant Cell Rep), published in Germany. (Language: eng)

Reference: 2004-Aug; vol 23 (issue 1-2) : pp 39-45

Dates: Created 2004/08/04; Completed 2004/10/27; Revised 2006/11/15;

PMID: 14997338, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

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