Immunodiagnosis of human fascioliasis using an antigen of Fasciola gigantica adult worm with the molecular mass of 27 kDa by a dot-ELISA.

Full Abstract

Immunodominant antigens of an approximate molecular mass of 27 kDa (FG 27) were obtained from an excretory-secretory product of adult Fasciola gigantica by a simple continuous-elution method. A dot-ELISA using the FG 27 antigen was developed for the detection of specific antibodies from patients infected with F. gigantica. Control sera were obtained from patients with other parasitic infections and healthy volunteers. The accuracy, sensitivity, specificity, and positive and negative predictive values were 98.2%, 100%, 97.4%, 76.9% and 100%, respectively. This dot-ELISA is a specific, sensitive and easy to perform method for the rapid diagnosis of fascioliasis, particularly when more complex laboratory tests are unavailable.

Author information

Author/s: Intapan, Pewpan M (PM); Maleewong, Wanchai (W); Nateeworanart, Saengchai (S); Wongkham, Chaisiri (C); Pipitgool, Vichit (V); Sukolapong, Varaporn (V); Sangmaneedet, Somboon (S);

Affiliation: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. pewpan(-atsign-)kku.ac.th

Journal and publication information

Publication Type: Clinical Trial; Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't

Journal: The Southeast Asian journal of tropical medicine and public health (Southeast Asian J Trop Med Public Health), published in Thailand. (Language: eng)

Reference: 2003-Dec; vol 34 (issue 4) : pp 713-7

Dates: Created 2004/04/29; Completed 2004/05/19; Revised 2006/11/15;

PMID: 15115076, status: MEDLINE (last retrieved date: 2/18/2009)

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