Sho-saiko-to prevents liver fibrosis induced by bile duct ligation in rats.

Full Abstract

Hepatic fibrosis is an over-accumulation of extracellular matrix (ECM). It is a result of an imbalance between collagen synthesis and degradation. Matrix metalloproteinase (MMP) has degradative activity against collagen, but tissue inhibitors of metalloproteinase (TIMP) control the active forms of MMP by blocking the active site of MMP. In our study, we established the bile duct ligated model (BDL) in rats to evaluate anti-fibrotic potential of Chinese medicine sho-saiko-to (TJ-9). We assessed the drug's potential in inhibiting collagen accumulation, suppressing procollagen alpha1 types (I) and (III), and TIMP-1 mRNA expression. After administration of TJ-9, hyperbilirubinemia reduced approximately four-fold when compared with BDL-untreated group. TJ-9 also significantly reduced the collagen content and fibrogenic score, as well as downregulated elevated procollagen alpha1 types (I) and (III) and TIMP-1 mRNA level. Finally, we concluded that (1) TJ-9 significantly reduced cholestasis in rats with BDL, (2) TJ-9 markedly reduced the collagen content by 50%, and (3) TJ-9 exerted its antifibrogenic effect by downregulation of the mRNA expression of procollagen alpha1 types (I) and (III), and TIMP-1 in liver tissue.

Author information

Author/s: Chen, Ming-Ho (MH); Chen, Jung-Chou (JC); Tsai, Chin-Chuan (CC); Wang, Wen-Chuang (WC); Chang, De-Ching (DC); Lin, Chun-Ching (CC); Hsieh, Hsiao-Yen (HY);

Affiliation: Research Institute of Chinese Medicine, a China Medical University, Taichung, Taiwan.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: The American journal of Chinese medicine (Am J Chin Med), published in United States. (Language: eng)

Reference: 2004-; vol 32 (issue 2) : pp 195-207

Dates: Created 2004/08/18; Completed 2004/11/02; Revised 2006/11/15;

PMID: 15315258, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Bile Ducts - surgery
Collagen - pharmacokinetics
Collagen Type I - biosynthesis
Collagen Type III - biosynthesis
Disease Models, Animal
Down-Regulation
Drugs, Chinese Herbal - pharmacology

Drugs, Chinese Herbal - pharmacology
Ligation
Liver Cirrhosis - prevention & control; veterinary
Male
RNA, Messenger - analysis
Rats
Rats, Wistar
Tissue Inhibitor of Metalloproteinase-1 - biosynthesis

Associated Chemicals: Collagen Type I (0) ; Collagen Type III (0) ; Drugs, Chinese Herbal (0) ; RNA, Messenger (0) ; Tissue Inhibitor of Metalloproteinase-1 (0) ; shosaiko-to (0) ; Collagen (9007-34-5)

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