A non-specific lipid transfer protein with antifungal and antibacterial activities from the mung bean.

Full Abstract

A non-specific lipid transfer peptide (nsLTP) with antimicrobial activity was isolated from the mung bean (Phaseolus mungo) seeds. The procedure entailed aqueous extraction, ion exchange chromatography on CM-Sephadex and high performance liquid chromatography (HPLC) on POROS-HS-20. The peptide exhibited a molecular mass of 9.03 kDa in mass spectrometry. It exerted antifungal action toward Fusarium solani, Fusarium oxysporum, Pythium aphanidermatum and Sclerotium rolfsii, and antibacterial action against Staphylococcus aureus but not against Salmonella typhimurium. The lipid binding of this peptide was very similar to that of a previously described lipid transfer protein extracted from wheat seeds and maize seeds, indicating that it possessed lipid transfer activity. The present findings add to the scarcity of the literature on leguminous nsLTPs.

Author information

Author/s: Wang, Shao Yun (SY); Wu, Jin Hong (JH); Ng, T B (TB); Ye, Xiu Yun (XY); Rao, Ping Fan (PF);

Affiliation: Insititute of Biotechnology, Fuzhou University, Fuzhou 350002, China.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Peptides (Peptides), published in United States. (Language: eng)

Reference: 2004-Aug; vol 25 (issue 8) : pp 1235-42

Dates: Created 2004/09/07; Completed 2005/02/10; Revised 2006/11/15;

PMID: 15350690, status: MEDLINE (last retrieved date: 2/18/2009)

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