A preliminary trial using multi-target polymerase chain reaction (multiplex PCR) and restriction fragment length polymorphism (PCR-RFLP) on the same feedstuffs to detect tissues of animal origin.

Full Abstract

A preliminary study using multi-target polymerase chain reaction (multiplex PCR) and restriction fragment length polymorphism (PCR-RFLP) was done on the same feedstuffs to detect animal tissues. The results of the two methods differ somewhat: PCR-RFLP did not detect any signal in any sample, but multiplex PCR detected a signal in one sample. These findings could be a basis for further investigations.

Author information

Author/s: Colombo, F (F); Marchisio, E (E); Trezzi, I E (IE); Peri, V (V); Pinotti, L (L); Baldi, A (A); Soncini, G (G);

Affiliation: Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza Alimentare, Milan, Italy. fabio.colombo(-atsign-)unimi.it

Journal and publication information

Publication Type: Evaluation Studies; Journal Article

Journal: Veterinary research communications (Vet Res Commun), published in Netherlands. (Language: eng)

Reference: 2004-Aug; vol 28 (issue 6) : pp 461-6

Dates: Created 2004/10/28; Completed 2004/12/08;

PMID: 15509020, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

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