Research article summary (published 13 Dec 2004):

Novel method for the authentication of frigate tunas (Auxis thazard and Auxis rochei) in commercial canned products.

Full Abstract

A novel procedure for the authentication of frigate tunas (Auxis thazard and Auxis rochei) in commercially canned products has been developed. Three mitochondrial regions were simultaneously amplified by multiplex-Polymerase Chain Reaction, one corresponding to the small rRNA 12S subunit as a positive amplification control and two species-specific fragments corresponding to cytochrome b for A. rochei and ATPase 6 for A. thazard, respectively. Testing of two different detection systems revealed the fluorescence-based approach as the most sensitive. The results demonstrate that this rapid, low-cost methodology is a reliable molecular tool for direct application in the authentication of canned products.

Author information

Author/s: Infante, Carlos (C); Catanese, Gaetano (G); Ponce, Marian (M); Manchado, Manuel (M);

Affiliation: Laboratorio de Identificación de Especies Pesqueras y Acuícolas, CIFPA El Toruño, IFAPA Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía, 11500 El Puerto de Santa María (Cádiz), Spain. carlos.infante.ext(-atsign-)juntadeandalucia.es

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of agricultural and food chemistry (J Agric Food Chem), published in United States. (Language: eng)

Reference: 2004-Dec; vol 52 (issue 25) : pp 7435-43

Dates: Created 2005/01/28; Completed 2005/04/20; Revised 2006/11/15;

PMID: 15675785, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Base Sequence Fish Products - analysis Food Preservation - standards Molecular Sequence Data

Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal - analysis Tuna - genetics

Associated Chemicals: RNA, Ribosomal (0) ; RNA, ribosomal, 12S (0)

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