Research article summary (published 13 May 2005):

Counteraction by repetitive daily exposure to static magnetism against sustained blockade of N-methyl-D-aspartate receptor channels in cultured rat hippocampal neurons.

Full Abstract

In rat hippocampal neurons cultured with the antagonist for N-methyl-D-aspartate (NMDA) receptors dizocilpine (MK-801) for 8 days in vitro (DIV), a significant decrease was seen in the expression of microtubule-associated protein-2 (MAP-2) as well as mRNA for both brain-derived neurotrophic factor (BDNF) and growth-associated protein-43 (GAP-43), in addition to decreased viability. MK-801 not only decreased the expression of the NR1 subunit of NMDA receptors but also increased NR2A expression, without affecting NR2B expression. Repetitive daily exposure to static magnetic fields at 100 mT for 15 min led to a decrease in the expression of MAP-2, without significantly affecting cell viability or the expression of neuronal nuclei (NeuN) and GAP-43. However, the repetitive magnetism prevented decreases in both BDNF mRNA and MAP-2 and additionally increased the expression of NR2A subunit, without altering NR1 expression in neurons cultured in the presence of MK-801. Repetitive magnetism was also effective in preventing the decrease by MK-801 in the ability of NMDA to increase intracellular free Ca2+ ions, without affecting the decrease in the maximal response. These results suggest that repetitive magnetism may at least in part counteract the neurotoxicity of MK-801 through modulation of the expression of particular NMDA receptor subunits in cultured rat hippocampal neurons.

Author information

Author/s: Hirai, Takao (T); Taniura, Hideo (H); Goto, Yasuaki (Y); Tamaki, Keisuke (K); Oikawa, Hirotaka (H); Kambe, Yuki (Y); Ogura, Masato (M); Ohno, Yu (Y); Takarada, Takeshi (T); Yoneda, Yukio (Y);

Affiliation: Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Graduate School of Natural Science and Technology, Kakuma-machi, Kanazawa, Ishikawa, Japan.

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of neuroscience research (J Neurosci Res), published in United States. (Language: eng)

Reference: 2005-May; vol 80 (issue 4) : pp 491-500

Dates: Created 2005/05/04; Completed 2005/07/25; Revised 2007/11/15;

PMID: 15846781, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Analysis of Variance Animals Blotting, Western - methods Brain-Derived Neurotrophic Factor - genetics; metabolism Calcium - metabolism Cell Survival - drug effects; radiation effects Cells, Cultured Dizocilpine Maleate - pharmacology Dose-Response Relationship, Drug Embryo, Mammalian Excitatory Amino Acid Antagonists - pharmacology Extracellular Space - drug effects; metabolism; radiation effects GAP-43 Protein - genetics; metabolism

Gene Expression Regulation - drug effects; physiology; radiation effects Hippocampus - cytology Magnetics Microtubule-Associated Proteins - genetics; metabolism N-Methylaspartate - pharmacology Neurons - drug effects; metabolism; radiation effects Phosphopyruvate Hydratase - metabolism RNA, Messenger - metabolism Rats Rats, Wistar Receptors, N-Methyl-D-Aspartate - genetics; metabolism Reverse Transcriptase Polymerase Chain Reaction - methods Time Factors

Associated Chemicals: Brain-Derived Neurotrophic Factor (0) ; Excitatory Amino Acid Antagonists (0) ; GAP-43 Protein (0) ; Microtubule-Associated Proteins (0) ; N-methyl D-aspartate receptor subtype 2A (0) ; RNA, Messenger (0) ; Receptors, N-Methyl-D-Aspartate (0) ; N-Methylaspartate (6384-92-5) ; Calcium (7440-70-2) ; Dizocilpine Maleate (77086-22-7) ; Phosphopyruvate Hydratase (EC 4.2.1.11)

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