[Effects of macrophage migration inhibitory factor on collagen I and III mRNA expression in vascular smooth muscle cells in vitro]

Full Abstract

OBJECTIVE: To investigate the association between macrophage migration inhibitory factor (MIF) and collagen synthesis in vascular smooth muscle cells (VSMCs). METHOD: VSMCs isolated from SD rat aorta were cultured in different culture plates, and the fourth passage of cells were divided into two groups, one of which was simulated by MIF and the other served as control group. Collagen I and III mRNA expression in the VSMCs was detected by reverse transcriptase (RT)-PCR and PCR. RESULT: Collagen I and III mRNA expression in MIF-treated VSMCs increased significantly as compared with that in the control cells (P<0.05). CONCLUSION: MIF increases collagen I and III mRNA expression of VSMCs in vitro, indicating its involvement in collagen synthesis in VSMCs.

Author information

Author/s: Li, Jun (J); Lin, Shu-guang (SG); Yu, Xi-yong (XY); Lin, Qiu-xiong (QX);

Affiliation: Institute of Cardiovascular Diseases, Guangdong People's Hospital, Guangzhou 510080, China. linzhongxiangma(-atsign-)sina.com

Journal and publication information

Publication Type: English Abstract; Journal Article; Research Support, Non-U.S. Gov't

Journal: Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA (Di Yi Jun Yi Da Xue Xue Bao), published in China. (Language: chi)

Reference: 2005-Jun; vol 25 (issue 6) : pp 684-6

Dates: Created 2005/06/16; Completed 2007/06/12;

PMID: 15958309, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

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