Research article summary (published 30 Jul 2005):

Adeno-associated virus-mediated bone morphogenetic protein-7 gene transfer induces C2C12 cell differentiation into osteoblast lineage cells.

Full Abstract

AIM: To investigate the effects of bone morphogenetic protein-7 (BMP7)-expressing recombinant adeno-associated virus (AAV) vector on the differentiation of C2C12 cells. METHODS: AAV-BMP7 was packaged by infecting the stable cell clone BHK-21 (integrated with recombinant AAV vector plasmid pSNAV-BMP7) with recombinant herpes simplex virus type 1, which expresses AAV-2 Rep and Cap and possesses AAV packaging functions. Following infection with AAV-BMP7 at multiplicities of infection of 1 x 10(5) vector genomes per cell and subsequent culture, C2C12 cells were assessed qualitatively for BMP7 production, alkaline phosphatase activity, osteocalcin production and Cbfal and MyoD expression. RESULTS: C2C12 cells transduced with AAV-BMP7 could produce BMP7 protein until d 28. Alkaline phosphatase in the cultured C2C12 cell lysate was elevated. Secreted osteocalcin in the culture medium was detectable at d 12 and Cbfal mRNA expression level was upregulated, coinciding with downregulation of MyoD in a temporal manner. CONCLUSION: The present in vitro study demonstrated that AAV-BMP7 could infect and efficiently convert C2C12 cells from myoblasts into osteoblast lineage cells.

Author information

Author/s: Yang, Min (M); Ma, Qing-jun (QJ); Dang, Geng-ting (GT); Ma, Kang-tao (KT); Chen, Ping (P); Zhou, Chun-yan (CY);

Affiliation: Department of Orthopedics, Peking University Third Hospital, Beijing 100083, China.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Acta pharmacologica Sinica (Acta Pharmacol Sin), published in China. (Language: eng)

Reference: 2005-Aug; vol 26 (issue 8) : pp 963-8

Dates: Created 2005/07/25; Completed 2006/05/03; Revised 2008/11/21;

PMID: 16038629, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Alkaline Phosphatase - metabolism Animals Bone Morphogenetic Protein 7 Bone Morphogenetic Proteins - biosynthesis; genetics; physiology Cell Differentiation Cell Line Cell Lineage Core Binding Factor Alpha 1 Subunit - genetics Dependovirus - genetics Enzyme-Linked Immunosorbent Assay Gene Expression

Genetic Vectors - genetics Humans MyoD Protein - genetics Myoblasts - cytology; metabolism Osteoblasts - cytology; metabolism Osteocalcin - biosynthesis Pluripotent Stem Cells - cytology; metabolism RNA, Messenger - genetics; metabolism Time Factors Transfection Transforming Growth Factor beta - biosynthesis; genetics; physiology

Associated Chemicals: BMP7 protein, human (0) ; Bone Morphogenetic Protein 7 (0) ; Bone Morphogenetic Proteins (0) ; Core Binding Factor Alpha 1 Subunit (0) ; MyoD Protein (0) ; RNA, Messenger (0) ; Transforming Growth Factor beta (0) ; Osteocalcin (104982-03-8) ; Alkaline Phosphatase (EC 3.1.3.1)

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