Research article summary (published 29 Jun 2005):

[Effects of gamma-interferon on gene expression of collagen I, III and on the tissue inhibitor of metalloprotenase 1 in HSC-T6 cells]

Full Abstract

OBJECTIVE: To observe the in vitro effects of gamma-interferon (IFNgamma) on gene expression of collagen I (Col I), III (Col III) and tissue inhibitor of metalloprotenase 1 (TIMP1) of HSC-T6 cells. METHODS: Cultured HSC-T6 cells were exposed to IFNgamma at concentrations of 0.1, 1, 10, 10(2), 10(3), 10(4), 2.5 x 10(5), 5 x10(5) U/ml for 48 hours. 4,5-simethylthiazaoly colormetric assay was used to evaluate the effect of IFNgamma on HSC-T6 cell proliferation. After incubating with IFNgamma (1 U/ml, 10(2) U/ml and 10(4) U/ml) for 48 hours, HSC-T6 cells were harvested to detect Col I, Col III and TIMP1 steady state mRNA levels by quantitative reverse-transcription polymerase chain reaction (RT-PCR). RESULTS: The Col I, Col III and TIMP1 mRNA levels of the control group were 2.86+/-0.21, 2.00+/-0.23 and 3.90+/-0.81, respectively. Col I and Col III mRNA levels in HSC-T6 cells treated by different concentrations of IFNgamma were lower than that of the controls (P < 0.01). There was no significant difference in TIMP1 mRNA levels between IFNgamma groups and controls. CONCLUSION: IFNgamma suppresses expression of Col I and Col III whereas it has no effect on TIMP1 mRNA expression. The antifibrotic mechanism of IFNgamma may be partly due to its down-regulation of Col I and Col III mRNA levels in HSC-T6 cells.

Author information

Author/s: Ma, Hong (H); Ma, Xue-mei (XM); Yin, Cheng-hong (CH); Jia, Ji-dong (JD); Wang, Bao-en (BE);

Affiliation: Liver Research Center, Beijing Friendship Hospital, Capital University of Medical Sciences, Beijing 100050, China. mahongmd(-atsign-)yahoo.com.cn

Journal and publication information

Publication Type: English Abstract; Journal Article; Research Support, Non-U.S. Gov't

Journal: Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (Zhonghua Gan Zang Bing Za Zhi), published in China. (Language: chi)

Reference: 2005-Jul; vol 13 (issue 7) : pp 528-30

Dates: Created 2005/07/26; Completed 2007/09/07; Revised 2008/11/21;

PMID: 16042892, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Cell Line Collagen Type I - biosynthesis; genetics Collagen Type III - biosynthesis; genetics Hepatocytes - cytology; metabolism

Interferon-gamma - pharmacology RNA, Messenger - biosynthesis; genetics Rats Rats, Sprague-Dawley Tissue Inhibitor of Metalloproteinase-1 - biosynthesis; genetics

Associated Chemicals: Collagen Type I (0) ; Collagen Type III (0) ; RNA, Messenger (0) ; Tissue Inhibitor of Metalloproteinase-1 (0) ; Interferon-gamma (82115-62-6)

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