Research article summary (published 17 Sep 2005):

Exogenous nitric oxide enhances the synthesis of type I collagen and heat shock protein 47 by normal human dermal fibroblasts.

Full Abstract

BACKGROUND: It is well established that the alterations of dermal matrix contributes to skin aging characterized by wrinkles. On the other hand, physiological NO is useful to maintain skin homeostasis such as a vasodilatation. However, a role of NO on production of dermal matrix has been clarified. OBJECTIVE: In this study, we have attempted to analyze the role of NO on type I collagen synthesis of normal human dermal fibroblasts including expression of procollagen alphaI S(1) mRNA/protein and heat shock protein 47 (HSP47). METHODS: The effects of NO which was generated by two types of NO donors, sodium nitroprusside (SNP) and S-nitroso-N-acetylpenicillamine (SNAP), on type I collagen and HSP47 and their related mRNA expression were examined with ELISA and RT-PCR. RESULTS: NO was significantly accelerated the production of type I collagen by fibroblasts corresponding with up-regulation of procollagen alphaI (1) mRNA. Furthermore, NO increased both levels of HSP47 protein and mRNA in fibroblasts in a dose-dependent manner. CONCLUSIONS: These results suggest that NO has dual effects on collagen synthesis by fibroblasts as follows; one is the direct stimulation of collagen synthesis due to the up-regulation of procollagen alphaI(1) mRNA, and the other is an indirect effect through the increase of HSP47 mRNA expression. This is the first report that exogenous NO stimulates HSP47 production by dermal fibroblasts.

Author information

Author/s: Obayashi, Kei (K); Akamatsu, Hirohiko (H); Okano, Yuri (Y); Matsunaga, Kayoko (K); Masaki, Hitoshi (H);

Affiliation: Cosmos Technical Center Co. Ltd., 3-24-3 Hasune, Itabashi-Ku, Tokyo 174-0046, Japan.

Journal and publication information

Publication Type: Journal Article

Journal: Journal of dermatological science (J Dermatol Sci), published in Netherlands. (Language: eng)

Reference: 2006-Feb; vol 41 (issue 2) : pp 121-6

Dates: Created 2006/01/18; Completed 2006/03/16;

PMID: 16171977, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Cells, Cultured Collagen Type I - analysis; biosynthesis; genetics Dermis - cytology; metabolism Dose-Response Relationship, Drug Enzyme-Linked Immunosorbent Assay Fibroblasts - chemistry; cytology; metabolism Gene Expression Regulation - drug effects HSP47 Heat-Shock Proteins - analysis; biosynthesis; genetics Humans

Humans Nitric Oxide - physiology Nitric Oxide Donors - pharmacology Nitroprusside - pharmacology Penicillamine - analogs & derivatives; pharmacology Procollagen - analysis; biosynthesis; genetics RNA, Messenger - analysis; genetics Reverse Transcriptase Polymerase Chain Reaction Up-Regulation - drug effects; physiology

Associated Chemicals: Collagen Type I (0) ; HSP47 Heat-Shock Proteins (0) ; Nitric Oxide Donors (0) ; Procollagen (0) ; RNA, Messenger (0) ; S-nitro-N-acetylpenicillamine (0) ; Nitric Oxide (10102-43-9) ; Nitroprusside (15078-28-1) ; Penicillamine (52-67-5)

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