Research article summary (published 30 Jan 1992):

Synergistic effects of dexamethasone on platelet-derived growth factor mitogenesis in vitro.

Full Abstract

Platelet-derived growth factor (PDGF) and insulin-like growth factor-I (IGF-I) interact to stimulate proliferation of fibroblasts in culture. Glucocorticoids variably effect the response of cultured fibroblasts to polypeptide growth factors. This study determined the effects of dexamethasone on growth-factor stimulation of gingival, periodontal ligament and pulp fibroblast proliferation in vitro. Cultures of quiescent, low-passage, human fibroblasts were treated for varying periods of time with transforming growth factor-beta 2 (TGF-beta 2), PDGF and IGF-I: (1) alone, (2) in combination with each other, (3) singly plus dexamethasone, (4) in combination plus dexamethasone. Combinations of human, recombinant PDGF and IGF-I (10-1000 ng/ml) induced significantly higher rates of cell proliferation than either factor alone. Dexamethasone at doses ranging from 10(-5) to 10(-8) M substantially enhanced cell proliferation induced by these combinations and by PDGF without IGF-I but not IGF-I alone. By 6 days after a single application, 2-3 times as many cells were present in the PDGF and dexamethasone cultures as compared to PDGF without IGF-I. TGF-beta 2 specifically blocked the effects of dexamethasone added to PDGF-stimulated cells. Collagen and non-collagenous protein synthesis was not affected by the addition of PDGF and IGF-I with or without dexamethasone. These data suggest that dexamethasone may substitute for IGF-I in PDGF stimulation of cell proliferation.

Author information

Author/s: Rutherford, R B (RB); TrailSmith, M D (MD); Ryan, M E (ME); Charette, M F (MF);

Affiliation: School of Dental Medicine, University of Connecticut, Farmington 06030.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Archives of oral biology (Arch Oral Biol), published in ENGLAND. (Language: eng)

Reference: 1992-Feb; vol 37 (issue 2) : pp 139-45

Dates: Created 1992/08/06; Completed 1992/08/06; Revised 2006/11/15;

PMID: 1622340, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Cell Division - drug effects Cells, Cultured Collagen - biosynthesis Dental Pulp - cytology; drug effects; metabolism Dexamethasone - administration & dosage; pharmacology Dose-Response Relationship, Drug Drug Synergism Fibroblasts - drug effects; metabolism Gingiva - cytology; drug effects; metabolism

Gingiva - cytology; drug effects; metabolism Humans Insulin-Like Growth Factor I - administration & dosage; pharmacology Mitogens - administration & dosage; pharmacology Periodontal Ligament - cytology; drug effects; metabolism Platelet-Derived Growth Factor - administration & dosage; pharmacology Proline - metabolism Prostaglandins E - biosynthesis Transforming Growth Factor beta - administration & dosage; pharmacology

Associated Chemicals: Mitogens (0) ; Platelet-Derived Growth Factor (0) ; Prostaglandins E (0) ; Transforming Growth Factor beta (0) ; Proline (147-85-3) ; Dexamethasone (50-02-2) ; Insulin-Like Growth Factor I (67763-96-6) ; Collagen (9007-34-5)

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